Evidence map›Paper›PMID 40770642›Full record

ArticleJournal of orthopaedic surgery and research2025

Influence of in vitro test conditions on the Biomechanical properties of degenerated human lateral menisci.

Michael Seidenstuecker, Luisa de Roy, Max F Weiske, O Piquet, Graciosa Quelhas Teixeira, Hermann O Mayr, Bianca Riedel, Andreas M Seitz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of orthopaedic surgery and research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Michael SeidenstueckerG.E.R.N. Research Center for Tissue Replacement, Regeneration & Neogenesis, Department of Orthopedics and Trauma Surgery, Faculty of Medicine, Medical Center-Albert- Ludwigs-University of Freiburg, Albert-Ludwigs-University of Freiburg, Hugstetter Straße 55, 79106, Freiburg, Germany.
Luisa de RoyInstitute of Orthopaedic Research and Biomechanics, Center of Trauma Research Ulm, Ulm University Medical Center, Helmholtzstr. 14, 89081, Ulm, Germany.
Max F WeiskeG.E.R.N. Research Center for Tissue Replacement, Regeneration & Neogenesis, Department of Orthopedics and Trauma Surgery, Faculty of Medicine, Medical Center-Albert- Ludwigs-University of Freiburg, Albert-Ludwigs-University of Freiburg, Hugstetter Straße 55, 79106, Freiburg, Germany.
O PiquetInstitute of Orthopaedic Research and Biomechanics, Center of Trauma Research Ulm, Ulm University Medical Center, Helmholtzstr. 14, 89081, Ulm, Germany.
Graciosa Quelhas TeixeiraInstitute of Orthopaedic Research and Biomechanics, Center of Trauma Research Ulm, Ulm University Medical Center, Helmholtzstr. 14, 89081, Ulm, Germany.
Hermann O MayrG.E.R.N. Research Center for Tissue Replacement, Regeneration & Neogenesis, Department of Orthopedics and Trauma Surgery, Faculty of Medicine, Medical Center-Albert- Ludwigs-University of Freiburg, Albert-Ludwigs-University of Freiburg, Hugstetter Straße 55, 79106, Freiburg, Germany.
Bianca RiedelG.E.R.N. Research Center for Tissue Replacement, Regeneration & Neogenesis, Department of Orthopedics and Trauma Surgery, Faculty of Medicine, Medical Center-Albert- Ludwigs-University of Freiburg, Albert-Ludwigs-University of Freiburg, Hugstetter Straße 55, 79106, Freiburg, Germany.
Andreas M SeitzInstitute of Orthopaedic Research and Biomechanics, Center of Trauma Research Ulm, Ulm University Medical Center, Helmholtzstr. 14, 89081, Ulm, Germany. andreas.seitz@uni-ulm.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The biomechanical properties of degenerated meniscal tissue are increasingly being studied in the context of osteoarthritis research. Spatial indentation testing using a multiaxial testing machine allows non-destructive characterization of viscoelastic properties. However, in vitro testing conditions can significantly influence the results. The purpose of this round robin study was to evaluate the effects of different fixation methods and laboratory environments on the viscoelastic properties of degenerated lateral menisci. Spatial normal indentation tests were performed on nine degenerated human lateral menisci in two laboratories using a multiaxial testing machine. Key parameters, including the maximum applied force (Pmax), instantaneous modulus (IM), and elastic modulus (Et10), were analyzed across different meniscus regions. Significant differences in the IM, Et10, and Pmax were observed between the laboratories, highlighting the influence of testing conditions on biomechanical results. The results indicated that variations in fixation methods, environmental conditions, and freeze-thaw cycles significantly affect the elastic and viscoelastic properties of meniscal tissue. Unphysiological strains in the inner region of the menisci suggested that strain-controlled indentation may be preferable to distance-controlled testing. These results underscore the importance of standardizing in vitro conditions for meaningful comparisons with the existing literature.

Indexed as

Mechanical TestsMenisci, TibialAgedBiomechanical PhenomenaElasticityElastic ModulusFemaleHumansIn Vitro TechniquesMaleMiddle AgedViscosityCollagenElastic modulusFixation methodsInstantaneous modulusMechanical examinationMeniscusOsteoarthritisSulfated glycosaminoglycan

Identifiers

PMID40770642
PMCPMC12326754

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.