Evidence map›Paper›PMID 40768581›Full record

ArticleScience advances2025

Integrated horizontal convective PCR system for clinical diagnostics.

Wenshang Guo, Minghui Xu, Ye Tao, Weiyu Liu, Hongwei Zhou, Xue Guan, Ruizhe Yang, Zhenyou Ge, Rui Xue, Zhongqiang Zhang and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Wenshang GuoState Key Laboratory of Robotics and System, Harbin Institute of Technology, Harbin 150001, China.ORCID 0009-0008-5613-3562
Minghui XuSchool of Mechatronics Engineering, Harbin Institute of Technology, Harbin 150001, China.ORCID 0009-0009-1148-3275
Ye TaoSchool of Mechatronics Engineering, Harbin Institute of Technology, Harbin 150001, China.ORCID 0000-0002-0092-3484
Weiyu LiuSchool of Electronics and Control Engineering, Chang'an University, Xi'an 710000, China.
Hongwei ZhouDepartment of Laboratory Diagnosis, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.ORCID 0009-0008-0717-9222
Xue GuanDepartment of Laboratory Diagnosis, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Ruizhe YangState Key Laboratory of Robotics and System, Harbin Institute of Technology, Harbin 150001, China.
Zhenyou GeState Key Laboratory of Robotics and System, Harbin Institute of Technology, Harbin 150001, China.ORCID 0000-0002-9492-3345
Rui XueSchool of Mechatronics Engineering, Harbin Institute of Technology, Harbin 150001, China.
Zhongqiang ZhangSchool of Mechanical Engineering, Jiangsu University, Zhenjiang 212013, China.ORCID 0000-0002-3485-777X
Haizhou ZhouDepartment of Laboratory Diagnosis, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.ORCID 0000-0002-2562-4229
Yukun RenState Key Laboratory of Robotics and System, Harbin Institute of Technology, Harbin 150001, China.ORCID 0000-0002-0167-1274

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rapid and accurate disease diagnosis in resource-limited settings is critical for optimizing medical resources and controlling epidemic outbreaks. However, developing a point-of-care detection system that is user-friendly, cost-effective, highly accurate, and exhibits strong specificity remains a formidable challenge. Here, we report an integrated horizontal convection PCR system (IHCS) for clinical point-of-care testing, capable of real-time fluorescence detection. IHCS leverages horizontal thermal convection within a capillary to drive sample flow and temperature cycling for nucleic acid amplification, making it suitable for both home use and on-site rapid detection. Capillary action enables swift sample loading, thereby simplifying the overall procedure, and the horizontal thermal convection overcomes the volume limitations inherent in vertical systems. Validation with 130 clinical samples demonstrated that the system successfully detected HBV, SARS-CoV-2, and influenza A virus within 40 min, achieving excellent sensitivity (>96%) and specificity (100%). Overall, the IHCS integrates microfluidics, thermal engineering, and optical sensing and offers a paradigm-shifting solution for point-of-care diagnostics.

Indexed as

COVID-19Polymerase Chain ReactionHepatitis B virusHumansInfluenza A virusPoint-of-Care SystemsPoint-of-Care TestingSARS-CoV-2Sensitivity and Specificity

Identifiers

PMID40768581
PMCPMC12327461

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.