Evidence map›Paper›PMID 40768485›Full record

ArticlePlant biotechnology journal2025

Hybridization Drives Trait Integration in Telomere-To-Telomere Apocynum Genomes.

Pan Xu, Fan Wu, Qi Yan, Bao Ao, Shengsheng Wang, Lijun Chen, Li Wang, Jiyu Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant biotechnology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Pan XuState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.ORCID https://orcid.org/0009-0009-4728-8260
Fan WuState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
Qi YanState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
Bao AoState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
Shengsheng WangState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
Lijun ChenState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
Li WangState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
Jiyu ZhangState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.ORCID https://orcid.org/0000-0002-3642-373X

Funding

Gansu Province Chief Scientist Responsibility Program (23ZDKA013) 23ZDKA013
6 · The paper itself

Abstract

Hybridization drives plant adaptation, yet its genomic mechanisms in non-model perennials remain elusive. Apocynum species thrive in extreme saline-alkaline environments. This study establishes A. pictum (APZ) as a homoploid hybrid of A. venetum (AVX) and A. hendersonii (AHG), exemplifying hybrid-driven resilience. Leveraging telomere-to-telomere (T2T) genome assemblies of AVX, APZ, and AHG, we confirmed APZ's hybrid origin ~0.95 million years ago (Mya), following species divergence ~2.08 Mya, with AHG plastid inheritance. Nuclear and plastid analyses resolve taxonomic disputes among three Apocynum species. APZ exhibits large heterozygous inversions on chromosomes 3 and 8 with suppressed recombination, preserving AHG stress-tolerance haplotypes. The study also showed that allele-specific expression (ASE) dynamically regulates salt tolerance: AHG-biased stress MAPK signalling pathway prevails at 200 mM NaCl, shifting to AVX-bias at 400 mM NaCl, while flavonoid biosynthesis genes such as AvFLS and AvCHS5 consistently favour AVX alleles. Transgenic assays validate AVX-derived AvFLS for superior salt tolerance and ROS scavenging, with AvCHS5 diversification driven by tandem duplication dosage effects. Homoploid hybrid speciation (HHS) analysis indicates AvCHS5 and circadian LHY genes under positive selection enhance hybrid stability, supporting breeding potential. This study reveals how hybridization drives trait integration via dynamic ASE, identifying AvFLS, AvCHS5, and stress-responsive loci as breeding targets for stress-resilient, flavonoid-rich cultivars, offering a genomic foundation for crop improvement in extreme environments.

Indexed as

Genome, PlantHybridization, GeneticTelomereSalt ToleranceApocynumASEflavonoid biosynthesishybrid speciationstress adaptationstructural variationT2T

Identifiers

PMID40768485
PMCPMC12576458

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.