Evidence map›Paper›PMID 40768179›Full record

ArticleApplied biochemistry and biotechnology2025

Comparative Study on Structural, Functional, and Biological Properties of Enzymatic Protein Hydrolysates from Different Legumes Native to Vietnam.

Thi-Thuy-Dung Nguyen, Thanh-Sang Nguyen, Thi-Van-Linh Nguyen, Thi-Tuong-Vi Tran, Quoc-Trung Huynh, Anh Duy Do, Quoc-Duy Nguyen

Abstract readComparative Study
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In one paragraph

Article in Applied biochemistry and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Thi-Thuy-Dung NguyenFaculty of Applied Science and Technology (FAST), Nguyen Tat Thanh University, Ho Chi Minh City, 700000, Vietnam.
Thanh-Sang NguyenFood Technology Faculty, Saigon Technology University, Ho Chi Minh City, 700000, Vietnam.
Thi-Van-Linh NguyenFaculty of Applied Science and Technology (FAST), Nguyen Tat Thanh University, Ho Chi Minh City, 700000, Vietnam.
Thi-Tuong-Vi TranFaculty of Applied Science and Technology (FAST), Nguyen Tat Thanh University, Ho Chi Minh City, 700000, Vietnam.
Quoc-Trung HuynhFaculty of Applied Science and Technology (FAST), Nguyen Tat Thanh University, Ho Chi Minh City, 700000, Vietnam.
Anh Duy DoCirTech Institute, HUTECH University, Ho Chi Minh City, 700000, Vietnam.
Quoc-Duy NguyenFaculty of Applied Science and Technology (FAST), Nguyen Tat Thanh University, Ho Chi Minh City, 700000, Vietnam. nqduy@ntt.edu.vn.ORCID http://orcid.org/0000-0001-9097-4471

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The growing demand for plant-based proteins reflects a consumer shift toward healthier and more sustainable lifestyles. Among these, hydrolyzed proteins have garnered attention due to their enhanced functional properties and biological activities compared to crude proteins. This study investigated the impact of enzymatic hydrolysis using Alcalase on the functional, technological, and biological properties of proteins derived from various beans, including yellow bean (Vigna radiata L.), hyacinth bean (Lablab purpureus L.), and lima bean (Phaseolus lunatus L.). The protein hydrolysates were assessed based on antioxidant activities (measured via ABTS radical scavenging and FRAP assays), foaming capacity, SDS-PAGE analysis, cytotoxicity, and anti-inflammatory properties. The results showed that the antioxidant activity of the protein hydrolysates significantly improved compared to the unhydrolyzed proteins, with hyacinth bean hydrolysates exhibiting the highest antioxidant activities, including ABTS radical scavenging activity (754.85 mg TE/L) and ferric reducing antioxidant power (80.35 mg TE/L). Meanwhile, yellow bean protein exhibited the highest degree of hydrolysis (81.38%) among the tested legumes and enzymatic hydrolysis significantly enhanced the foaming properties of yellow bean protein hydrolysates. SDS-PAGE analysis revealed that hydrolysis degraded plant-derived proteins into low-molecular-weight peptides (approximately 15-45 kDa). Additionally, the protein hydrolysates showed no cytotoxicity at concentrations below 2000 μg/mL. Legume protein hydrolysates hold significant future prospects in their promising potential as multifunctional ingredients exhibiting strong antioxidant and functional activities, which can be harnessed to develop sustainable, health-promoting food products and nutraceuticals.

Indexed as

FabaceaeProtein HydrolysatesSubtilisinsAnti-Inflammatory AgentsAntioxidantsHydrolysisVietnamAnti-Inflammatory AgentsAntioxidantsProtein HydrolysatesSubtilisinsBiological activitiesFunctional propertiesLegumeProtein hydrolysate

Identifiers

PMID40768179

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