Evidence map›Paper›PMID 40767967›Full record

ArticleMikrochimica acta2025

Integrated microfluidic cartridge for rapid and colorimetric detection of Mycoplasma pneumoniae.

Jinling Cui, Binghui Shi, Mengnan Chai, Yanling Wang, Jiansen Du, Xiaoguang Lu, Chao Shi, Cuiping Ma, Yong Li

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jinling Cui *College of Biological Engineering, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, Qingdao Key Laboratory of Rapid Nucleic Acid Detection, Qingdao Rapid Nucleic Acid Detection Engineering Research Center, Qingdao University of Science and Technology, Qingdao, 266042, China.
Binghui Shi *College of Biological Engineering, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, Qingdao Key Laboratory of Rapid Nucleic Acid Detection, Qingdao Rapid Nucleic Acid Detection Engineering Research Center, Qingdao University of Science and Technology, Qingdao, 266042, China.
Mengnan ChaiCollege of Biological Engineering, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, Qingdao Key Laboratory of Rapid Nucleic Acid Detection, Qingdao Rapid Nucleic Acid Detection Engineering Research Center, Qingdao University of Science and Technology, Qingdao, 266042, China.
Yanling WangCollege of Biological Engineering, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, Qingdao Key Laboratory of Rapid Nucleic Acid Detection, Qingdao Rapid Nucleic Acid Detection Engineering Research Center, Qingdao University of Science and Technology, Qingdao, 266042, China.
Jiansen DuQingdao International Travel Healthcare Center, Qingdao Customs District, Qingdao, 266071, China.
Xiaoguang LuCollege of Biological Engineering, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, Qingdao Key Laboratory of Rapid Nucleic Acid Detection, Qingdao Rapid Nucleic Acid Detection Engineering Research Center, Qingdao University of Science and Technology, Qingdao, 266042, China.
Chao ShiQingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life Sciences, Department of Pathogenic Biology, School of Basic Medicine, the Clinical Laboratory Department of the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, China.
Cuiping MaCollege of Biological Engineering, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, Qingdao Key Laboratory of Rapid Nucleic Acid Detection, Qingdao Rapid Nucleic Acid Detection Engineering Research Center, Qingdao University of Science and Technology, Qingdao, 266042, China.
Yong LiCollege of Biological Engineering, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, Qingdao Key Laboratory of Rapid Nucleic Acid Detection, Qingdao Rapid Nucleic Acid Detection Engineering Research Center, Qingdao University of Science and Technology, Qingdao, 266042, China. liyong_55@126.com.

Funding

Shandong Provincial Natural Science Foundation ZR2023QD125, ZR2023MC166
6 · The paper itself

Abstract

Mycoplasma pneumoniae is a major cause of community-acquired pneumonia, yet its diagnosis remains challenging due to overlapping symptoms with other respiratory pathogens. In this study, an integrated microfluidic cartridge platform has been developed that combines three synergistic advancements: a chemical lysis reagent (GuHCl/EDTA) enabling direct genomic DNA release from M. pneumoniae collected by nasal swabs within 2 min without extraction steps, a novel dual-dye system (hydroxy naphthol blue/SYBR Green I) that leverages complementary fluorescence transitions from red to green for enhanced visual discrimination of loop-mediated isothermal amplification (LAMP) results, and a smartphone-based RGB quantification algorithm to standardize interpretation by analyzing colorimetric changes. The microfluidic cartridge integrates lysis, amplification, and detection into a closed workflow, achieving a sample-to-answer time of 40 min with a detection limit of 1 copy/μL. Clinical validation using 25 nasal swab samples demonstrated 95% sensitivity (19/20) and 100% specificity (5/5) compared with serological tests. This platform offers a rapid, cost-effective, and user-friendly solution for point-of-care diagnosis of M. pneumoniae, particularly in resource-limited settings.

Indexed as

ColorimetryLab-On-A-Chip DevicesMicrofluidic Analytical TechniquesMycoplasma pneumoniaeDNA, BacterialHumansLimit of DetectionMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesPneumonia, MycoplasmaSmartphoneDNA, BacterialColorimetric detectionExtraction-free analysisLoop-mediated isothermal amplificationMicrofluidic cartridgeMycoplasma pneumoniaePOCT

Identifiers

PMID40767967

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.