ArticlebioRxiv : the preprint server for biology2025
Brr2p-mediated unwinding of U4/U6 is promoted by a mutually exclusive intra-molecular stem loop in U4 and involves destabilization of the 5' stem-loop of U4.
Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Before the spliceosome engages a pre-mRNA to excise its introns, the catalytic small nuclear RNA (snRNA) U6 is inactive because of base pairing with U4 snRNA; thus, spliceosome activation requires unwinding of base paired U4/U6, composed of stem I and stem II. The Ski2-like ATPase and RNA helicase Brr2p facilitates U4/U6 unwinding and the ultimately irreversible release of U4; however, the molecular mechanism behind Brr2p-mediated U4/U6 unwinding and the roles of the snRNAs in unwinding remains incompletely understood. To investigate the mechanism in vivo in budding yeast, we screened an unwinding deficient, cold-sensitive
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