Evidence map›Paper›PMID 40766509›Full record

ArticlebioRxiv : the preprint server for biology2025

Assessing cellular metabolic dynamics with NAD(P)H fluorescence polarization imaging.

Lu Ling, Jack C Crowley, Matthew L Tan, Jennie A M R Kunitake, Adrian A Shimpi, Rebecca M Williams, Lara A Estroff, Claudia Fischbach, Warren R Zipfel

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. The role of NADFrontiers in oncology · 2026
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Lu LingNancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York, 14853, USA.ORCID 0000-0001-6693-8665
Jack C CrowleySchool of Applied and Engineering Physics Cornell University, Ithaca NY, 14853, USA.ORCID 0000-0003-4220-8152
Matthew L TanNancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York, 14853, USA.ORCID 0000-0002-0176-8615
Jennie A M R KunitakeDepartment of Materials Science and Engineering, Cornell University, Ithaca, New York 14850, United States.ORCID 0000-0002-7061-3211
Adrian A ShimpiNancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York, 14853, USA.ORCID 0000-0002-0782-0496
Rebecca M WilliamsInstitute of Biotechnology, Cornell University, Ithaca, NY 14853, USA.
Lara A EstroffDepartment of Materials Science and Engineering, Cornell University, Ithaca, New York 14850, United States.ORCID 0000-0002-7658-1265
Claudia FischbachNancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York, 14853, USA.ORCID 0000-0002-9368-0150
Warren R ZipfelNancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York, 14853, USA.ORCID 0000-0003-2640-329X

Funding

Project 3: Physical and Metabolic Constraints of Cancer Cell InvasionU54CA210184 · NCI · CORNELL UNIVERSITY · PI SCHAFFER, CHRIS B · 2016 to 2020
$10.2M
Mechanical properties of adipose tissue and its effect on breast cancerR01CA276392 · NCI · CORNELL UNIVERSITY · PI Claudia Fischbach, Corey O'Hern · 2023 to 2026
$2.1M
Metabolic regulation of exosome biogenesis as a determinant of cancer cell metastasis.R01CA259195 · NCI · CORNELL UNIVERSITY · PI MARC A ANTONYAK, Claudia Fischbach · 2022 to 2026
$2.1M
Volumetric time-lapse imaging of biophysical cell-extracellular matrix interactions for systems mechanobiology researchR01GM132823 · NIGMS · CORNELL UNIVERSITY · PI ADIE, STEVEN GRAHAM · 2019 to 2022
$1.7M
Broad wavelength range Zeiss 780 NLO/confocal system for the Cornell Imaging CoreS10OD018516 · OD · CORNELL UNIVERSITY · PI ZIPFEL, WARREN R · 2014 to 2014
$834k
NCI NIH HHS R01 CA259195NCI NIH HHS R01 CA276392NCI NIH HHS U54 CA210184NIGMS NIH HHS R01 GM132823NIH HHS S10 OD018516
6 · The paper itself

Abstract

Altered metabolism enables adaptive advantages for cancer, driving the need for improved methods for non-invasive long-term monitoring of cellular metabolism from organelle to population level. Here we present two-photon steady-state fluorescence polarization ratiometric microscopy (FPRM), a label-free imaging method that uses nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) autofluorescence as a functional readout of cellular metabolism. The method is simple to implement and operates an order of magnitude faster than the NAD(P)H-fluorescence lifetime imaging microscopy (FLIM) imaging modality, reducing cytotoxic stress while providing long-term monitoring capacity. FPRM enables high-resolution dynamic tracking of NAD(P)H signals with subcellular details and we have established a set of instrument-independent ratiometric parameters that correlates NAD(P)H signals with metabolic status during pharmaceutical and environmental perturbations. We further integrated FPRM readouts with other parameters such as cell shape and migration on 2D and 3D collagen matrices, demonstrating the technique's versatility across bioengineered platforms for cancer metabolism research.

Indexed as

cancerMetabolismNADHtwo-photon microscopy

Identifiers

PMID40766509
PMCPMC12324316

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.