Evidence map›Paper›PMID 40766472›Full record

ArticlebioRxiv : the preprint server for biology2025

Maternal CENP-C restores centromere symmetry in mammalian zygotes to ensure proper chromosome segregation.

Catherine A Tower, Gabriel Manske, Emily L Ferrell, Dilara N Anbarci, Kelsey Jorgensen, Binbin Ma, Mansour Aboelenain, Rajesh Ranjan, Saikat Chakraborty, Lindsay Moritz and 9 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

19 authors.

Catherine A TowerDepartment of Human Genetics, University of Michigan, Ann Arbor, MI.ORCID 0000-0003-4470-6227
Gabriel ManskeDepartment of Human Genetics, University of Michigan, Ann Arbor, MI.ORCID 0000-0001-5461-4647
Emily L FerrellDepartment of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI.ORCID 0009-0008-9721-3868
Dilara N AnbarciDepartment of Human Genetics, University of Michigan, Ann Arbor, MI.ORCID 0000-0003-0435-4749
Kelsey Jorgensen. Department of Anthropology, University of Kansas, Lawrence, KS.ORCID 0000-0002-8681-5248
Binbin MaHoward Hughes Medical Institute, Department of Biology, John Hopkins University, Baltimore, MD.ORCID 0009-0003-1048-4459
Mansour AboelenainDepartment of Genetics, Rutgers University, Piscataway, NJ.ORCID 0000-0002-3806-6748
Rajesh RanjanHoward Hughes Medical Institute, Department of Biology, John Hopkins University, Baltimore, MD.ORCID 0000-0003-1368-6067
Saikat ChakrabortyDepartment of Human Genetics, University of Michigan, Ann Arbor, MI.ORCID 0000-0002-2908-7205
Lindsay MoritzDepartment of Human Genetics, University of Michigan, Ann Arbor, MI.ORCID 0000-0001-6704-8453
Arunika DasDepartment of Biomedical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY.ORCID 0000-0001-7116-3489
Michele BoianiMax Planck Institute for Molecular Biomedicine, Department of Cell & Tissue Dynamics, Muenster, Germany.ORCID 0000-0003-2765-2781
Ben E BlackDepartment of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA.ORCID 0000-0002-3707-9483
Shawn ChavezDivision of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR.ORCID 0000-0002-8285-0222
Erica E MarshDepartment of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI.ORCID 0000-0003-1331-6525
Ariella ShikanovCellular and Molecular Biology Graduate Program, University of Michigan, Ann Arbor, MI.ORCID 0000-0003-0159-6419
Karen SchindlerDepartment of Genetics, Rutgers University, Piscataway, NJ.ORCID 0000-0001-6113-1184
Xin ChenHoward Hughes Medical Institute, Department of Biology, John Hopkins University, Baltimore, MD.ORCID 0000-0002-6990-0926
Saher Sue HammoudDepartment of Human Genetics, University of Michigan, Ann Arbor, MI.ORCID 0000-0002-5040-6370

Funding

PREDOCTORAL TRAINING IN GENETICST32GM007544 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MORAN, JOHN V. · 1985 to 2022
$11.3M
Cellular and Molecular Biology at MichiganT32GM145470 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI John Chadwick Brenner · 2022 to 2026
$4.1M
Study epigenetic inheritance during development, homeostasis and regenerationR35GM127075 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Xin Chen · 2018 to 2026
$3.2M
Age and molecular mechanisms contributing to aneuploidy in oocytesR01HD058730 · NICHD · UNIVERSITY OF PENNSYLVANIA · PI BLACK, BEN E., LAMPSON, MICHAEL · 2009 to 2020
$3.2M
Signaling Mechanisms that Control Chromosome Segregation during Female Meiosis (Equipment Administrative Supplement)R35GM136340 · NIGMS · RUTGERS, THE STATE UNIV OF N.J. · PI Karen A Schindler · 2020 to 2026
$3.1M
Contributions of Sperm Chromatin to development: A Myth or Reality?DP2HD091949 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HAMMOUD, SAHER SUE · 2016 to 2016
$2.3M
Sperm Chromatin: Implications on organismal development and fertilityR01HD104680 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HAMMOUD, SAHER SUE · 2021 to 2024
$1.8M
The Role of Parental Histone Proteins in Early DevelopmentF31HD100124 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MANSKE, GABRIEL LEE · 2019 to 2021
$113k
Balancing Act: Equalizing Sex Specific Differences in CENP-A levels is Critical for Mammalian EmbryogenesisF31HD117648 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Catherine Tower · 2025 to 2026
$87k
NICHD NIH HHS DP2 HD091949NICHD NIH HHS F31 HD100124NICHD NIH HHS F31 HD117648NICHD NIH HHS R01 HD058730NICHD NIH HHS R01 HD104680NIGMS NIH HHS R35 GM127075NIGMS NIH HHS R35 GM136340NIGMS NIH HHS T32 GM007544NIGMS NIH HHS T32 GM145470
6 · The paper itself

Abstract

Across metazoan species, the centromere-specific histone variant CENP-A is essential for accurate chromosome segregation, yet its regulation at the parental-to-zygote transition in mammals is poorly understood. To address this, we developed a CENP-A-mScarlet knock-in mouse model, which revealed sex-specific dynamics: mature sperm retains 10% of the CENP-A levels present in MII-oocytes. However, in zygotes prior to the first mitosis, this difference is resolved, using maternally inherited cytoplasmic-CENP-A. Notably, the increase in CENP-A at paternal centromeres is independent of sensing CENP-A asymmetry or the presence of maternal chromosomes. Instead, CENP-A equalization relies on asymmetric recruitment of maternal CENP-C to paternal centromeres. Depletion of maternal CENP-A decreases total CENP-A in pronuclei without disrupting equalization. In contrast, reducing maternal CENP-C or disruption of its dimerization domains impairs CENP-A equalization and chromosome segregation. Therefore, maternal CENP-C acts a key epigenetic regulator that resets centromeric symmetry at fertilization to preserve genome integrity.

Indexed as

CENP-ACENP-CCentromereepigeneticsintergenerationalmouseoocytespermzygote

Identifiers

PMID40766472
PMCPMC12324218

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.