Evidence map›Paper›PMID 40766316›Full record

ArticleFrontiers in immunology2025

Advanced immunophenotyping of lymphocyte and monocyte subsets in healthy Australian adults using a novel spectral flow cytometry panel.

Ainsley R Davies, Kristy Kwong, Zhijia Yu, Koula E M Diamand, Fei-Ju Li, Laurensia Kannitha, Sidra A Ali, Abolfazl Amjadipour, Ann-Maree Padarin, Michael Devoy and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Ainsley R DaviesCanberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.
Kristy KwongCanberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.
Zhijia YuCanberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.
Koula E M DiamandPhenomics Translation Initiative, John Curtin School of Medical Research, Australian Phenomics Facility, The Australian National University, Canberra, ACT, Australia.
Fei-Ju LiPhenomics Translation Initiative, John Curtin School of Medical Research, Australian Phenomics Facility, The Australian National University, Canberra, ACT, Australia.
Laurensia KannithaCanberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.
Sidra A AliDivision of Genome Science and Cancer, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.
Abolfazl AmjadipourDivision of Immunology and Infectious Disease, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.
Ann-Maree PadarinCanberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.
Michael DevoyCytometry, Histology and Advanced Spatial Multiomics, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.
Harpreet VohraCytometry, Histology and Advanced Spatial Multiomics, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.
Bahar MiraghazadehDivision of Immunology and Infectious Disease, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.
Simon H JiangDivision of Immunology and Infectious Disease, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.
Anne BrüstleDivision of Immunology and Infectious Disease, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.
Nicolas CherbuinNational Centre for Epidemiology and Population Health, Australian National University, Canberra, ACT, Australia.
Christopher J NolanDivision of Immunology and Infectious Disease, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.
Matthew C CookDepartment of Medicine, University of Cambridge, Cambridge, United Kingdom.
Elizabeth E GardinerDivision of Genome Science and Cancer, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.
Stuart ReadPhenomics Translation Initiative, John Curtin School of Medical Research, Australian Phenomics Facility, The Australian National University, Canberra, ACT, Australia.
Euan McNaughtonCanberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.
Katrina L RandallCanberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Lymphocytes play pivotal roles in disease pathogenesis and can be used as potential biomarkers for various immunological conditions. Yet, current flow cytometry methods used in clinical settings are often only capable of measuring between four to eight distinct lymphocyte populations. The purpose of our study was to measure many lymphocyte and monocyte populations from a single sample, with the long-term aim of validating our assay for diagnostic use in the Australian regulatory environment. Methods: We designed and optimised a novel 30-colour lymphocyte immunophenotyping panel tailored for use on a 3-laser (V-B-R) spectral flow cytometer. This panel measures over 50 lymphocyte and monocyte populations. Results: In this report we present data derived from 148 healthy individuals. Discussion: This lays the groundwork for future clinical application of spectral flow cytometry tests and offers a more comprehensive approach to lymphocyte and monocyte analysis with future implications for disease diagnosis and monitoring.

Indexed as

Flow CytometryImmunophenotypingLymphocytesLymphocyte SubsetsMonocytesAdultAgedAustraliaFemaleHealthy VolunteersHumansMaleMiddle AgedYoung AdultautoimmunityB cellimmunophenotypinglymphocytePBMCspectral flow cytometryT cell

Identifiers

PMID40766316
PMCPMC12322900

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.