Evidence map›Paper›PMID 40766230›Full record

ArticleResearch square2025

Targeted RNA sequencing identified gene expression profiles linked to severe necrosis and mortality in preterm infants with surgical necrotizing enterocolitis.

Parvesh Mohan Garg, David Sawaya, Robin Riddick, Seth Lirette, Nicole Hall, Neha Varshney, Timothy D Howard, William B Hillegass, Akhil Maheshwari, Padma Garg

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Parvesh Mohan GargDepartment of Pediatrics/Neonatology, Wake Forest University School of Medicine, Winston Salem, North Carolina.ORCID 0000-0001-5949-7304
David SawayaDepartment of Pediatric Surgery, University of Mississippi Medical Center, Jackson, Mississippi.
Robin RiddickDepartment of Pediatrics, University of Mississippi Medical Center, Jackson, Mississippi.
Seth LiretteDepartment of Biostatistics, University of Mississippi Medical Center, Jackson, Mississippi.
Nicole HallDepartment of Biochemistry, Wake Forest University School of Medicine, Winston Salem, North Carolina.
Neha VarshneyDepartment of Pathology, University of Mississippi Medical Center, Jackson, Mississippi.
Timothy D HowardDepartment of Biochemistry, Wake Forest University School of Medicine, Winston Salem, North Carolina.
William B HillegassDepartment of Biostatistics, University of Mississippi Medical Center, Jackson, Mississippi.
Akhil MaheshwariNeonatology, Boston Children's Physicians Group at New York Medical College and Maria Ferrari Children's Hospital, Westchester, NY.
Padma GargDepartment of Pediatrics, University of Mississippi Medical Center, Jackson, Mississippi.

Funding

Training and Mentoring Core P20GM103476 · NIGMS · UNIVERSITY OF SOUTHERN MISSISSIPPI · PI MICHAEL R GARRETT · 2012 to 2026
$60.2M
Tracking and Evaluation CoreU54GM115428 · NIGMS · UNIVERSITY OF MISSISSIPPI MED CTR · PI MASON, SARAH · 2016 to 2025
$38.2M
Role of obesity in preeclamptic pregnancy.P20GM121334 · NIGMS · UNIVERSITY OF MISSISSIPPI MED CTR · PI Pier Paolo Claudio, Babbette LaMarca · 2017 to 2026
$26.4M
Sex differences in operant cocaine memoriesP20GM144041 · NIGMS · UNIVERSITY OF MISSISSIPPI MED CTR · PI Ramona Moles · 2023 to 2026
$12.1M
Pilot Projects ProgramP30GM149404 · NIGMS · UNIVERSITY OF MISSISSIPPI MED CTR · PI John E Hall · 2023 to 2026
$6.3M
NIGMS NIH HHS P20 GM103476NIGMS NIH HHS P20 GM121334NIGMS NIH HHS P20 GM144041NIGMS NIH HHS P30 GM149404NIGMS NIH HHS U54 GM115428
6 · The paper itself

Abstract

Background: We aim to determine the gene expression changes that occur in surgical NEC infants with and without moderate to severe necrosis and survivors and non-survivors. Methods: Targeted RNA sequencing was performed on RNA isolated from formalin-fixed, paraffin-embedded (FFPE) intestinal tissue samples (N=36). DeSeq2 was used to analyze differential expressions between infants with mild to moderate and severe necrosis and with respect to survival status. Results: Thirty-five genes were differentially expressed (FDR- adjusted p < 0.05) between mild-medium necrosis and severe necrosis. Genes involved in altered host defense, natural killer (NK) cell signaling and development, and apoptosis were overexpressed in severe necrosis ( Conclusion: Severe necrosis and non-survival of NEC infants were associated with differential genes expression related to host defense, NK cell signaling and development, and apoptosis. Understanding these pathways can guide the development of prognostic and treatment pathways.

Indexed as

Gene ExpressionMortalityNECNecrosisNeonatePreterm InfantsRNA sequencing

Identifiers

PMID40766230
PMCPMC12324612

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.