Specific reaction conditions for efficient automated

Introduction: Automated radiolabeling of gallium-68-labeled experimental radiopharmaceuticals is crucial for ensuring high reproducibility and regulatory compliance in clinical settings. FAP-2286, a promising DOTA-pseudopeptide targeting the tumor microenvironment, has demonstrated superior tumor retention compared to quinoline-based analogs, making it an attractive theranostic agent. This study aimed to optimize and automate the preparation of [ Methods: Manual radiolabeling assays were initially performed to identify optimal reaction conditions, varying buffer, antioxidant, vector amount, heating time, and purification methods. The selected conditions were then adapted to an automated protocol using a GAIA Results: Initial optimization studies identified sodium acetate buffer 0.1 M with methionine as an antioxidant, 25 μg of FAP-2286, and a 4-min heating time as the best manual radiolabeling conditions, achieving a RCP > 98%. In the automated synthesis, adjustments were made, including doubling the vector amount and extending heating to 9 min, resulting over three test-batches in a moderate RCY of 59.85 ± 3.73% and a RCP just over 94% up to 4 h after the end of synthesis. Importantly, the method was successfully transposed to [ Conclusion: This study established a robust, automated protocol for the synthesis of [