ArticleStem cell research & therapy2025
Harnessing extracellular vesicles from adipose-derived stem cells for the treatment of 4-vinylcyclohexene diepoxide-induced premature ovarian insufficiency.
Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed.
- Mesenchymal stem cell-based therapeutic strategies for female infertility: current evidence and emerging targeting approaches.Biotechnology letters · 2026Review
- miR-379-5p promotes ovarian granulosa cell apoptosis in primary ovarian insufficiency by targeting KNDC1 and PEG10.Frontiers in genetics · 2026Article
- Recent advances in methods for establishing animal models of premature ovarian failure.Journal of ovarian research · 2025Review
- Mesenchymal stem cells and extracellular vesicles for knee osteoarthritis: clinical application, mechanism exploration and prospect.Stem cell research & therapy · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors.
Funding
Abstract
backgroundPremature ovarian insufficiency (POI) is a challenging condition with limited effective treatments. Adipose-derived stem cells (ADSCs) have demonstrated potential in tissue repair, and their extracellular vesicles (ADSC-EVs) show more safety in clinical translation. However, the role and mechanism of ADSC-EVs in the treatment of POI are not yet fully understood. This study aims to investigate the protective effects of ADSC-EVs on rat POI models induced by 4-vinylcyclohexene diepoxide (VCD) and to explore the potential therapeutic mechanisms.
methodsRat ADSCs and ADSC-EVs were isolated and characterized. The POI rat model was established via intraperitoneal injection of VCD for 15 consecutive days. ADSCs and ADSC-EVs were injected into the ovaries for treatment. Ovary function was assessed by monitoring estrous cycles, follicle counts, sexual hormone levels, and ovulation. Molecular mechanisms were investigated using TUNEL staining, immunohistochemistry, quantitative polymerase chain reaction, and western blotting. In vitro, primary rat granulosa cells were treated with VCD in the presence or absence of ADSC-EVs. Cell proliferative ability, hormone secretion, apoptosis rate, and relative molecular expression were measured. Whole-transcriptome sequencing and DIA proteomics of ADSC-EVs were performed to identify bioactive molecules.
resultsADSC-EVs protected granulosa cells from VCD-induced apoptosis, promoted cell proliferation, enhanced hormone secretion, and upregulated KITL expression. Treatment with ADSCs and ADSC-EVs in POI rats significantly improved estrous cycles, follicle counts, and serum levels of estradiol (E2), follicle-stimulating hormone (FSH), and anti-Müllerian hormone (AMH). These treatments activated the KITL/KIT/PI3K/AKT signaling pathway, downregulated pro-apoptotic genes (Bax, Caspase3), and upregulated anti-apoptosis genes (Bcl2). ADSC-EVs are highly enriched in mRNA of Kit and PI3K, and both transcriptomic and proteomics enrichment analysis predominantly focused on PI3K/AKT pathway.
conclusionsADSCs and ADSC-EVs effectively protect and restore ovarian function in VCD-induced POI rats. The mechanism involves inhibiting apoptosis in granulosa cells and activating the KITL/KIT/PI3K/AKT pathway in ovary. ADSC-EVs, with advantages in clinical translation, hold significant potential for POI treatment.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.