Evidence map›Paper›PMID 40764385›Full record

ArticleNature communications2025

Functional avidity of anti-B7H3 CAR-T constructs predicts antigen density thresholds for triggering effector function.

Marta Barisa, Henrike P Muller, Elisa Zappa, Rivani Shah, Juliane L Buhl, Benjamin Draper, Courtney Himsworth, Chantelle Bowers, Sophie Munnings-Tomes, Marilena Nicolaidou and 28 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Article
  2. Engineering CAR-T cells to remodel the mucin-rich cancer cell glycocalyx.bioRxiv : the preprint server for biology · 2026
    Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. CAR-T cell therapy targeting MUC17 in gastric tumors.Journal for immunotherapy of cancer · 2025
    Article
  12. Review
  13. Article
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

38 authors.

Marta BarisaGreat Ormond Street Institute of Child Health, University College London, London, UK. m.barisa@ucl.ac.uk.ORCID http://orcid.org/0000-0001-7846-6810
Henrike P Muller *Great Ormond Street Institute of Child Health, University College London, London, UK.ORCID http://orcid.org/0000-0002-4682-4553
Elisa Zappa *Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.
Rivani ShahGreat Ormond Street Institute of Child Health, University College London, London, UK.
Juliane L BuhlPrincess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.
Benjamin DraperGreat Ormond Street Institute of Child Health, University College London, London, UK.
Courtney HimsworthGreat Ormond Street Institute of Child Health, University College London, London, UK.ORCID http://orcid.org/0000-0002-4233-7512
Chantelle BowersGreat Ormond Street Institute of Child Health, University College London, London, UK.
Sophie Munnings-TomesGreat Ormond Street Institute of Child Health, University College London, London, UK.
Marilena NicolaidouGreat Ormond Street Institute of Child Health, University College London, London, UK.ORCID http://orcid.org/0009-0001-5306-8960
Sonia MorlandoGreat Ormond Street Institute of Child Health, University College London, London, UK.
Kathleen BirleyGreat Ormond Street Institute of Child Health, University College London, London, UK.
Clara Leboreiro-BabeGreat Ormond Street Institute of Child Health, University College London, London, UK.ORCID http://orcid.org/0000-0003-4630-095X
Alice VitaliGreat Ormond Street Institute of Child Health, University College London, London, UK.
Laura PriviteraGreat Ormond Street Institute of Child Health, University College London, London, UK.
Kyle O'SullivanGreat Ormond Street Institute of Child Health, University College London, London, UK.
Ailsa GreppiGreat Ormond Street Institute of Child Health, University College London, London, UK.
Magdalena BuschhausLumicks, Amsterdam, The Netherlands.
Mario Barrera RománPrincess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.ORCID http://orcid.org/0000-0002-5919-2962
Sam de BlankPrincess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.ORCID http://orcid.org/0000-0003-2191-5675
Femke van den HamPrincess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.
Brenna R van 't VeldPrincess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.
Gabrielle FerryGreat Ormond Street Institute of Child Health, University College London, London, UK.
Jonathan FisherGreat Ormond Street Institute of Child Health, University College London, London, UK.ORCID http://orcid.org/0000-0003-3302-2241
Debarati ShomeLumicks, Amsterdam, The Netherlands.
Reza NadafiLumicks, Amsterdam, The Netherlands.
Israrul H AnsariDepartment of Pediatrics, University of Wisconsin School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
Rogier ReijmersLumicks, Amsterdam, The Netherlands.ORCID http://orcid.org/0000-0001-7471-7715
Stefano GiulianiGreat Ormond Street Institute of Child Health, University College London, London, UK.
Paul SondelDepartment of Pediatrics, University of Wisconsin School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
Laura K DonovanGreat Ormond Street Institute of Child Health, University College London, London, UK.ORCID http://orcid.org/0000-0001-5557-5539
Louis CheslerCancer Institute, University College London, London, UK.ORCID http://orcid.org/0000-0001-7842-2068
Jan MolenaarPrincess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.
Jarno DrostPrincess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.ORCID http://orcid.org/0000-0002-2941-6179
Anne C RiosPrincess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.ORCID http://orcid.org/0000-0002-9082-8068
Kerry ChesterThe Institute of Cancer Research, London, UK.ORCID http://orcid.org/0000-0002-3093-3130
Judith WienkePrincess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands. j.wienke-4@prinsesmaximacentrum.nl.ORCID http://orcid.org/0000-0002-3835-6374
John AndersonGreat Ormond Street Institute of Child Health, University College London, London, UK. j.anderson@ucl.ac.uk.ORCID http://orcid.org/0000-0001-7509-3203

Funding

DH | National Institute for Health Research (NIHR) GOSHBRCEC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020) 956285 (VAGABOND)
6 · The paper itself

Abstract

Chimeric Antigen receptor T cell (CAR-T) treatments for solid cancers have been compromised by limited expansion and survival in the tumor microenvironment following interaction with antigen-expressing target cells. Using B7H3 as a model antigen with broad clinical applicability, we evaluate the relationship between the antibody/antigen affinity of three clinical candidate binders and the three following characteristics: cellular avidity, duration of sustained cytotoxicity in tumoroid re-stimulation assays, and in vivo anti-tumoral responses. Next, BEHAV3D video microscopy is used to assess CAR-T cell interaction with tumor cells at single cell resolution. These data are consistent with a threshold avidity of CAR-T / tumor cell interaction and target cell B7H3 expression level, where enhanced functionality is characterized by longer cumulative CD8

Indexed as

B7 AntigensImmunotherapy, AdoptiveNeoplasmsReceptors, Chimeric AntigenAnimalsCD8-Positive T-LymphocytesCell Line, TumorFemaleHumansMiceTumor MicroenvironmentXenograft Model Antitumor AssaysB7 AntigensReceptors, Chimeric Antigen

Identifiers

PMID40764385
PMCPMC12325614

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.