Evidence map›Paper›PMID 40764374›Full record

ArticleScientific reports2025

Utilizing native nanodiscs to isolate active TRPC3 channels and expand structural analysis capabilities.

Jasmin Baron, Lena Bauernhofer, Sandro Keller, Franz P W Radner, Carolyn Vargas, Gerd Leitinger, Lukas Bernauer, Anita Emmerstorfer-Augustin, Patrick Wiedner, Grégory Durand and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. The role of IPPflugers Archiv : European journal of physiology · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Jasmin BaronGottfried Schatz Research Center, Division of Medical Physics and Biophysics, Medical University of Graz, Graz, Austria. jasmin.baron@medunigraz.at.
Lena BauernhoferBiophysics, Institute of Molecular Biosciences (IMB), NAWI Graz, University of Graz, Graz, Austria.
Sandro KellerBiophysics, Institute of Molecular Biosciences (IMB), NAWI Graz, University of Graz, Graz, Austria.
Franz P W RadnerField of Excellence BioHealth, University of Graz, Graz, Austria.
Carolyn VargasBiophysics, Institute of Molecular Biosciences (IMB), NAWI Graz, University of Graz, Graz, Austria.
Gerd LeitingerBioTechMed-Graz, Graz, Austria.
Lukas BernauerInstitute of Molecular Biotechnology, Graz University of Technology, Graz, Austria.
Anita Emmerstorfer-AugustinBioTechMed-Graz, Graz, Austria.
Patrick WiednerGottfried Schatz Research Center, Division of Medical Physics and Biophysics, Medical University of Graz, Graz, Austria.
Grégory DurandAvignon Université, UPRI, Avignon Cedex 9, 84916, France.
Marine SouliéAvignon Université, Chem2staB, Avignon Cedex 9, 84916, France.
Victoria DorrerInstitute of Chemical Technologies and Analytics, Technische Universität Wien, Getreidemarkt 9, Vienna, 1060, Austria.
Matthias SchittmayerInstitute of Chemical Technologies and Analytics, Technische Universität Wien, Getreidemarkt 9, Vienna, 1060, Austria.
Ruth Birner-GruenbergerInstitute of Chemical Technologies and Analytics, Technische Universität Wien, Getreidemarkt 9, Vienna, 1060, Austria.
Michaela LichteneggerGottfried Schatz Research Center, Division of Medical Physics and Biophysics, Medical University of Graz, Graz, Austria.
Matthias GsellGottfried Schatz Research Center, Division of Medical Physics and Biophysics, Medical University of Graz, Graz, Austria.
Klaus GroschnerGottfried Schatz Research Center, Division of Medical Physics and Biophysics, Medical University of Graz, Graz, Austria.
Rainer SchindlGottfried Schatz Research Center, Division of Medical Physics and Biophysics, Medical University of Graz, Graz, Austria.
Oleksandra TiapkoGottfried Schatz Research Center, Division of Medical Physics and Biophysics, Medical University of Graz, Graz, Austria. oleksandra.tiapko@medunigraz.at.

Funding

Austrian Science Fund 10.55776/PAT8197124Austrian Science Fund 5359 NAustrian Science Fund P33263Austrian Science Fund P35291Austrian Science Fund SFB F73BioTechMed-Graz LabRotationBioTechMed-Graz Stemp
6 · The paper itself

Abstract

Recent advances in structural biology have provided insights into TRPC3, a TRP family member involved in various (patho)physiological processes. However, the lack of structural information on the channel's open pore hampers understanding of its function and therapeutic potential. Cryogenic electron microscopy holds promise for elucidating TRPC3's open-pore conformation, but challenges remain in isolating it without compromising function. Our study evaluated novel extraction agents in comparison to conventional detergents for isolating functional TRPC3 complexes from HEK293, Komagataella phaffii, and Expi293F cells, identifying Expi293F as optimal for TRPC3 expression. Among the extraction agents screened, dodecyl diglucoside (DDDG) and n-dodecyl-β-D-maltoside (DDM) were the most effective for extracting TRPC3. We successfully purified TRPC3 under native conditions, preserving its tetrameric structure and activity, as confirmed by electron microscopy, mass spectrometry and patch-clamp analysis. This study highlights the importance of extraction agents in advancing TRPC3 research and therapeutic development.

Indexed as

NanostructuresTRPC Cation ChannelsCryoelectron MicroscopyHEK293 CellsHumansTRPC3 cation channelTRPC Cation ChannelsElectrophysiologyNanodiscsProtein purificationReconstitutionTRPC3

Identifiers

PMID40764374
PMCPMC12325699

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.