ArticleCell death discovery2025
Bridging the marrow: a co-culture-platform of leukemia cells and MS5-derived stromal cells or adipocytes.
Article in Cell death discovery, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Response to "Co-Culture Shock" by Behan & Mittelman: Co-culture shock? Let's integrate!Cell death discovery · 2026Article
- Co-culture shock.Cell death discovery · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
In the context of acute lymphoblastic leukemia (ALL), the bone marrow microenvironment (BMM) plays a crucial role in providing pro-survival signals, as evident from the rapid spontaneous cell death observed in ex-vivo cultures of primary ALL cells. Among the diverse cell types within the BMM, bone marrow adipocytes (BMAd) exhibit significant plasticity and can constitute a substantial part of the BMM, especially during ALL therapy. However, conflicting data on the interaction between ALL cells and adipocytes have been reported, potentially arising from variations in culture systems and conditions. Our study aimed to establish a chemically defined co-culture system of leukemia cells combined with either bone marrow (BM)-derived stromal cells or adipocytes. Established B-precursor ALL cell lines, patient-derived ALL xenografts (PDX), and murine BM-derived MS5 stromal cells and adipocytes were used as model systems. Fetal calf serum and factors included in the adipogenic media significantly impacted leukemia cell viability and proliferation. Thus, we implemented a washing procedure and suitable, chemically defined media conditions into our co-culture platform. In general, ALL cell lines survived and proliferated on both stromal cells and adipocytes, whereas PDX cells exhibited a pronounced survival advantage on stromal cells compared to adipocytes. Intriguingly, the presence of adipocytes sensitized cell lines and PDX cells to chemotherapy with anthracyclines or dexamethasone when compared to co-cultures with stromal cells. Thus, utilizing the well-established MS5 cell line together with the optimized culture conditions in our co-culture system provides a reliable, open-access platform for investigating intricate interactions between bone marrow stromal cells, adipocytes, and leukemia cells.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.