ReviewBiochemistry2025
Considering Metabolic Context in Enzyme Evolution and Design.
Review in Biochemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Short-term aerobic pretreatment of swine manure enhances short-chain fatty acid production in anaerobic fermentation: performance and mechanisms.Bioprocess and biosystems engineering · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Enzymes are often treated as isolated molecular entities when measuring their biochemical activity and designing synthetic sequences. Yet inside the cell, enzymes interact through shared metabolite pools, physical binding, and regulatory feedback. This coordination is necessary for responsive, cohesive metabolic behavior. Moreover, these interactions place constraints on enzyme activity, specificity, abundance, and ultimately sequence. Defining these interaction-mediated constraints is important to understand metabolic evolution and to design synthetic systems. For example, the influence of the cellular and environmental milieu becomes evident when a heterologous enzyme from one species fails to function properly upon transfer to a new host. In this review we consider how cell context shapes enzyme evolution, and in turn, how variations in enzyme biochemistry, biophysics, and abundance impact cell fitness.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.