Evidence map›Paper›PMID 40762828›Full record

ArticleTropical animal health and production2025

Molecular detection of bovine coronavirus in the environment of a naturally infected dairy cattle herd.

Carla Regina Costa Furlan, Alais Maria Dall Agnol, Ana Paula Souza Frucchi, Carla Ivane Ganz Vogel, Rüdiger Daniel Ollhoff, Amauri Alcindo Alfieri, Cristina Santos Sotomaior

Abstract read
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In one paragraph

Article in Tropical animal health and production, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Carla Regina Costa FurlanGraduate Program in Animal Science, School of Medicine and Life Sciences, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, Paraná, Brazil.
Alais Maria Dall AgnolLaboratory of Animal Virology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
Ana Paula Souza FrucchiLaboratory of Animal Virology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
Carla Ivane Ganz VogelCenter for Agroveterinary Sciences, Universidade do Estado de Santa Catarina, Lages, Santa Catarina, Brazil.
Rüdiger Daniel OllhoffGraduate Program in Animal Science, School of Medicine and Life Sciences, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, Paraná, Brazil.
Amauri Alcindo AlfieriLaboratory of Animal Virology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
Cristina Santos SotomaiorGraduate Program in Animal Science, School of Medicine and Life Sciences, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, Paraná, Brazil. cristina.sotomaior@pucpr.br.ORCID http://orcid.org/0000-0001-9281-3743

Funding

National Institute of Science and Technology of Dairy Production Chain (CNPq/INCT-Leite) 465725/2014-7
6 · The paper itself

Abstract

Bovine coronavirus (BCoV) causes neonatal diarrhea and respiratory diseases in cattle. Detection of BCoV on surfaces and fomites in dairy facilities is rarely reported. We examined the presence of BCoV on various surfaces of a dairy farm with previous BCoV-positive cattle. A total of 61 samples were collected, including 37 samples of surfaces and fomites from the housing of suckling calves, weaned calves, heifers, and lactating cows. Fecal samples (n = 13) and nasal secretions (n = 11) were collected from calves and cows. Molecular identification of BCoV was performed using semi-nested RT-PCR. Amplicons of BCoV genes N and S1 from three positive samples (environment, diarrheic feces, and nasal secretions) were selected for nucleotide (nt) sequencing analysis. BCoV RNA was detected in 27% (10/37) of environmental samples, including wooden surfaces, concrete and tiles, plastic feed troughs, drinkers, and calf bottles. Furthermore, BCoV RNA was amplified in 46.1% of fecal and 36.4% of nasal secretion samples. Analysis of the N gene revealed 100% nt similarity between strains from different sources, while the S1 gene showed 99.7 to 99.8% similarity. The same BCoV strain was detected in cattle and on the evaluated surfaces. These strains were ancestrally different from the prototype strains but clustered with other Brazilian field strains. In conclusion, BCoV is present in cattle and on different surfaces within the environment of the evaluated dairy cattle farm. Biosecurity measures, such as cleaning and disinfection, are crucial to mitigate infection risk, especially for calves.

Indexed as

Cattle DiseasesCoronavirus, BovineCoronavirus InfectionsAnimalsCattleDairyingFecesFemaleFomitesHousing, AnimalPhylogenyRNA, ViralRNA, ViralBiosecurityCoronavirusDairyEnvironmental contaminationPneumoenteric virus

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.