Evidence map›Paper›PMID 40762489›Full record

ArticlemBio2025

A large-scale screening campaign of putative carbohydrate-active enzymes reveals a novel xylanase from anaerobic gut fungi.

Shiyan Jin, Isabella R Farrand, Yan Chen, Jennifer W Gin, Bo Zhang, Elaine Kirschke, Christopher J Petzold, Paul D Adams, Michelle A O'Malley

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mBio, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Shiyan JinDepartment of Chemical Engineering, University of California, Santa Barbara, California, USA.ORCID 0009-0005-1547-4107
Isabella R FarrandDepartment of Chemical Engineering, University of California, Santa Barbara, California, USA.
Yan ChenBiological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Jennifer W GinBiological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Bo ZhangDepartment of Chemical Engineering, University of California, Santa Barbara, California, USA.
Elaine KirschkeDepartment of Chemical Engineering, University of California, Santa Barbara, California, USA.
Christopher J PetzoldBiological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Paul D AdamsJoint BioEnergy Institute, Emeryville, California, USA.
Michelle A O'MalleyDepartment of Chemical Engineering, University of California, Santa Barbara, California, USA.ORCID 0000-0002-6065-8491

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The genomes of anaerobic gut fungi (AGF) encode a diverse array of carbohydrate-active enzymes (CAZymes), yet exceedingly few of these enzymes have been experimentally validated or expressed in heterologous systems. Here, we developed a predictive bioinformatic pipeline to annotate novel putative CAZymes from anaerobic fungi and validate their activity through large-scale heterologous expression in

Indexed as

Endo-1,4-beta XylanasesFungal ProteinsGastrointestinal MicrobiomePiromycesAnaerobiosisComputational BiologyEscherichia coliKineticsProteomicsRecombinant ProteinsSubstrate SpecificityXylansEndo-1,4-beta XylanasesFungal ProteinsRecombinant ProteinsXylansanaerobic fungicarbohydrate-active enzymesenzyme characterizationheterologous expressionprotein function predictionscreening

Identifiers

PMID40762489
PMCPMC12421816

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.