Evidence map›Paper›PMID 40762057›Full record

ArticleCirculation research2025

Dicer Neosynthesis Regulates Platelet Reactivity: A Mechanism Altered in Type 2 Diabetes.

Loredana Bury, Elisa Piselli, Giorgia Manni, Stefania Momi, Giulia Ciarrocca Taranta, Giuseppe Guglielmini, Carmine Fanelli, Michelantonio De Fano, Jesse W Rowley, Andrew S Weyrich and 1 more

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In one paragraph

Article in Circulation research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Loredana BuryDivision of Internal and Cardiovascular Medicine (L.B., E.P., G.M., S.M., G.C.T., G.G., P.G.), University of Perugia, Italy.ORCID 0000-0003-0333-0948
Elisa PiselliDivision of Internal and Cardiovascular Medicine (L.B., E.P., G.M., S.M., G.C.T., G.G., P.G.), University of Perugia, Italy.
Giorgia ManniDivision of Internal and Cardiovascular Medicine (L.B., E.P., G.M., S.M., G.C.T., G.G., P.G.), University of Perugia, Italy.ORCID 0000-0002-5697-2648
Stefania MomiDivision of Internal and Cardiovascular Medicine (L.B., E.P., G.M., S.M., G.C.T., G.G., P.G.), University of Perugia, Italy.
Giulia Ciarrocca TarantaDivision of Internal and Cardiovascular Medicine (L.B., E.P., G.M., S.M., G.C.T., G.G., P.G.), University of Perugia, Italy.
Giuseppe GuglielminiDivision of Internal and Cardiovascular Medicine (L.B., E.P., G.M., S.M., G.C.T., G.G., P.G.), University of Perugia, Italy.ORCID 0000-0002-8678-8518
Carmine FanelliDivision of Endocrino-Metabolic Sciences, Department of Medicine and Surgery (C.F., M.D.F.), University of Perugia, Italy.ORCID 0000-0003-4063-158X
Michelantonio De FanoDivision of Endocrino-Metabolic Sciences, Department of Medicine and Surgery (C.F., M.D.F.), University of Perugia, Italy.ORCID 0000-0002-2657-5991
Jesse W RowleyDepartment of Internal Medicine, University of Utah School of Medicine, Salt Lake City (J.W.R., A.S.W.).ORCID 0000-0003-4444-1724
Andrew S WeyrichDepartment of Internal Medicine, University of Utah School of Medicine, Salt Lake City (J.W.R., A.S.W.).
Paolo GreseleDivision of Internal and Cardiovascular Medicine (L.B., E.P., G.M., S.M., G.C.T., G.G., P.G.), University of Perugia, Italy.ORCID 0000-0001-5365-8445

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDespite being anucleate, platelets contain mRNAs and synthesize new proteins. Platelets also contain microRNAs and Dicer (ribonuclease III enzyme Dicer-1), an enzyme required for microRNA maturation. The expression of Dicer and some microRNAs is reduced in platelets from patients with type 2 diabetes (T2DM). However, the role of Dicer in the regulation of platelet function and in T2DM-associated platelet hyperreactivity is unclear. We aimed to assess whether Dicer levels are regulated in platelets upon activation, if they modulate mRNA translation by triggering pre-microRNA maturation, and whether these mechanisms are deranged in T2DM platelets.

methodsDicer expression in ultrapurified platelets was assessed by Western blotting, flow cytometry, and liquid chromatography-tandem mass spectrometry. P2Y

resultsThrombin-activated platelets from healthy individuals rapidly neosynthesize Dicer, leading to increased maturation of microRNA-223 and concomitant consumption of pre-microRNA-223. An increase in microRNA-223 was associated with a reduction of P2RY12 mRNA, one of its main targets, and platelet P2Y

conclusionsThe Dicer-triggered decrease in P2Y

Indexed as

Blood PlateletsDEAD-box RNA HelicasesDiabetes Mellitus, Type 2Platelet ActivationRibonuclease IIIAnimalsDiabetes Mellitus, ExperimentalFemaleHumansMaleMiceMice, Inbred C57BLMice, KnockoutMicroRNAsMiddle AgedReceptors, Purinergic P2Y12DEAD-box RNA HelicasesDICER1 protein, humanDicer1 protein, mouseMicroRNAsMIR223, humanP2RY12 protein, humanReceptors, Purinergic P2Y12Ribonuclease IIIThrombingene expressionmegakaryocytesmicroRNAsplatelet activation

Identifiers

PMID40762057
PMCPMC12398351

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.