ArticleArchives of clinical and biomedical research2025
Isolation of Primary Brain Cells: Challenges and Solutions.
Article in Archives of clinical and biomedical research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed.
- Evidence-Based Interventions for Functional Restoration and Pain Management following Traumatic Brain Injury.Archives of clinical and medical case reports · 2026Article
- From mice to clinical relevance: humanizing neuroscience with human-based model systems.Frontiers in cellular neuroscience · 2026Review
- Neurons in a Dish: A Review of In Vitro Cell Models for Studying Neurogenesis.Journal of neurochemistry · 2026Review
- Catechins in stroke: multi-target mechanisms, preclinical evidence, and translational challenges.Frontiers in pharmacology · 2026Review
- A cell line-derived, immune-competent neurospheroid model to study neuroinflammation and human brain disorders.Frontiers in immunology · 2026Article
- Novel advanced patient-derivedFrontiers in neurologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The isolation of primary brain cells is essential for studying cellular behavior, signaling pathways, and disease mechanisms in the central nervous system. This paper explores the general and specific steps involved in extracting and culturing neurons, astrocytes, and microglia from brain tissue, highlighting how primary cells maintain their functionality and structural integrity without genetic modification like immortalized cell lines. Marker proteins such as MAP-2, GFAP, IBA-1, and TMEM119 help confirm cell identity and allow tracking of phenotypic changes, such as inflammation or maturation. We critically discussed some technological problems that researchers usually face during extraction and culturing procedures, emphasizing that each brain source and particular cell type require strict conditions to maximize cellular yield and viability. Environmental control of the cells in culture, such as pH, CO
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.