Evidence map›Paper›PMID 40761184›Full record

ArticleLower urinary tract symptoms2025

Pharmacological Targeting of Hyperpolarization-Activated Cyclic Nucleotide-Gated Cation Channels on Bladder Afferent Sensory Transmission.

Eric Woon, Jorge Villalobos Santeli, Jia Liu, Longtu Chen, George Kuchel, Bin Feng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Lower urinary tract symptoms, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Eric WoonDepartment of Biomedical Engineering, University of Connecticut, Storrs, Connecticut, USA.
Jorge Villalobos SanteliDepartment of Biomedical Engineering, University of Connecticut, Storrs, Connecticut, USA.
Jia LiuDepartment of Biomedical Engineering, University of Connecticut, Storrs, Connecticut, USA.
Longtu ChenDepartment of Biomedical Engineering, University of Connecticut, Storrs, Connecticut, USA.
George KuchelUConn Center on Aging, UConn Health, Farmington, Connecticut, USA.ORCID https://orcid.org/0000-0001-8387-7040
Bin FengDepartment of Biomedical Engineering, University of Connecticut, Storrs, Connecticut, USA.ORCID https://orcid.org/0000-0001-9277-211X

Funding

Research Education ComponentP30AG067988 · NIA · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI Richard H Fortinsky, GEORGE A KUCHEL · 2021 to 2026
$9.5M
Detrusor Underactivity as an HCN-mediated Failure of Resilience in AgingR01AG058814 · NIA · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI KUCHEL, GEORGE A, LEVINE, ERIC S · 2019 to 2023
$3.1M
The Role of Lumbar Splanchnic Innervations in Visceral Nociception and PainR01DK120824 · NIDDK · UNIVERSITY OF CONNECTICUT STORRS · PI FENG, BIN · 2019 to 2023
$2.0M
NIA NIH HHS P30 AG067988NIA NIH HHS R01 AG058814NIDDK NIH HHS R01 DK120824NIH HHS AG058814, DK120824
6 · The paper itself

Abstract

objectiveThis study aimed to investigate the potential role of cesium chloride (CsCl), ivabradine (IVA), and isoproterenol (ISO) on the sensory transmission of bladder afferents to graded urinary bladder distension (UBD). We specifically selected these drugs to target the hyperpolarization-activated cyclic nucleotide-gated (HCN) cation channels to determine their role in afferent encoding.

methodsThe bladders of C57BL/6 female mice were harvested with attached pelvic nerves in continuity, and the stimulus-response function (SRF) of bladder afferents to stepped bladder distension (20, 40, 60, 80 cmH

resultsIVA and ISO did not significantly reduce afferent responses to UBD, whereas CsCl increased afferent responses. Bladder afferents in the pelvic nerve pathway were categorized into low-firing (LF, < 10 Hz) and high-firing (HF, > 10 Hz) groups. SRF in both the LF and HF groups showed similar trends with no significant changes in response to IVA and ISO. CsCl increased SRF only in the HF group but not in the LF group. Immunohistological staining revealed that HCN1 does not extensively co-localize with afferent endings, showing only sporadic presence.

conclusionOur targeted pharmacological studies with single-fiber recordings and immunohistological staining collectively suggest that HCN channels do not play a significant role in bladder afferent sensory transmission.

Indexed as

Hyperpolarization-Activated Cyclic Nucleotide-Gated ChannelsIsoproterenolNeurons, AfferentUrinary BladderAfferent PathwaysAnimalsCesiumChloridesFemaleIvabradineMiceMice, Inbred C57BLCesiumcesium chlorideChloridesHyperpolarization-Activated Cyclic Nucleotide-Gated ChannelsIsoproterenolIvabradinehyperpolarization‐activated cyclic nucleotide‐gated channelsivabradinenerve recordingurinary bladder

Identifiers

PMID40761184
PMCPMC12322805

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.