Evidence map›Paper›PMID 40760419›Full record

ArticleBMC genomics2025

Bta-novel-miR-25336 enhanced adipose differentiation and unsaturated fatty acid synthesis by targeting ACADS in intramuscular adipocytes of river buffalo.

Ruirui Zhu, Fangfang Zou, Hao Tian, Hongfang Mo, Qilin Chen, Xinyu Zeng, Shan Han, Deshun Shi, Jianghua Shang, Jieping Huang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ruirui Zhu *Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530005, China.
Fangfang Zou *Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530005, China.
Hao TianGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530005, China.
Hongfang MoGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530005, China.
Qilin ChenGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530005, China.
Xinyu ZengGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530005, China.
Shan HanGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530005, China.
Deshun ShiGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530005, China.
Jianghua ShangGuangxi Key Laboratory of Buffalo Genetics, Reproduction and Breeding, Buffalo Research Institute, Nanning, Guangxi, 530001, China.
Jieping HuangGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530005, China. huangjieping@gxu.edu.cn.

Funding

the National Natural Science Foundation of Guangxi province and the Postdoctoral Science Foundation of China 2024JJD130103 and 2024MD763953
6 · The paper itself

Abstract

backgroundThe intramuscular fat content and the level of unsaturated fatty acids influence meat quality and nutritional value. microRNAs can participate in lipid metabolic processes. Understanding how novel miRNAs regulate lipogenesis and fatty acid metabolism is key to enhancing buffalo meat quality and nutritional value.

resultsIn this study, miRNA sequencing was conducted on 36 adipose tissues from six anatomical sites in river buffaloes (Murrah). A total of 1,682 miRNAs were identified in 36 adipose tissues, of which 987 were novel and 695 were known miRNAs. Weighted gene co-expression networks analysis (WGCNA) revealed that bta-novel-miR-25336 was highly expressed in sternum subcutaneous adipose tissue (SSAT) and had a high correlation with unsaturated fatty acids (UFA). Functional studies showed that bta-novel-miR-25336 was positively correlated with the proliferation and adipogenic differentiation of both buffalo preadipocytes and intramuscular adipocytes. Additionally, we used bioinformatics analysis and a 3' untranslated region (3'UTR) luciferase reporter assay to validate acyl-CoA dehydrogenase short chain (ACADS) as a target gene of bta-novel-miR-25336.

conclusionsIn summary, bta-novel-miR-25336 may regulate biological processes associated with cell proliferation, lipid differentiation and unsaturated fatty acid synthesis through ACADS. This study provides a theoretical basis for further elucidating the regulation of lipid metabolism by novel miRNAs in farm animals.

Indexed as

AdipocytesAdipogenesisBuffaloesFatty Acids, UnsaturatedMicroRNAs3' Untranslated RegionsAdipose TissueAnimalsCell Differentiation3' Untranslated RegionsFatty Acids, UnsaturatedMicroRNAsAdipogenic differentiationBubalus bubalisMiRNAUnsaturated fatty acid

Identifiers

PMID40760419
PMCPMC12323136

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.