ArticleAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry2025
Light-up RNA aptamer-based T-NASBA: a one-pot, label-free strategy for miRNA detection.
Article in Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Electrochemical analysis of miRNA for liquid biopsy.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2026Article
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5 authors.
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Abstract
MicroRNAs (miRNAs) are pivotal regulators of cellular processes, with dysregulation linked to diverse diseases, particularly cancer. Current methods, while effective, are often expensive, complex, and require sophisticated equipment. To address these limitations, we developed a label-free, one-pot miRNA detection platform integrating toehold-mediated strand displacement (TMSD) with nucleic acid sequence-based amplification (NASBA). This method utilizes a TMSD-regulated hairpin Primer1 that selectively responds to target miRNA, triggering NASBA to generate a light-Up RNA aptamer for real-time, target-dependent fluorescence signaling-eliminating the need for fluorophore-labeled probes or additional modifications. By systematic optimization of the reaction conditions, the platform achieved a detection limit of 4.31 pM, with a strong linear correlation between fluorescence intensity and miRNA concentration over a range from 10 pM to 1 nM. The platform also demonstrated excellent reproducibility and precision in spike-and-recovery tests with human serum, with recovery rates between 99.85
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