Evidence map›Paper›PMID 40758195›Full record

ReviewPlant cell reports2025

A review of factors affecting the success of geminivirus infectious clones.

S R Möller, M N Maruthi

Abstract readReview
In one paragraph

Review in Plant cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

S R MöllerUniversity of Greenwich, Natural Resources Institute, Medway, Kent, UK. S.R.Moller@greenwich.ac.uk.ORCID http://orcid.org/0000-0003-0392-4631
M N MaruthiUniversity of Greenwich, Natural Resources Institute, Medway, Kent, UK.

Funding

HORIZON EUROPE European Innovation Council 101000570
6 · The paper itself

Abstract

key messageIn this review, we provide a summary of factors that affect the successful infection of geminivirus clones in plants to enable the greater understanding of plant-virus interactions. Geminiviruses are single-stranded DNA viruses that can cause significant losses in economically important crops worldwide. Considerable efforts have been made to study the geminiviruses in detail, which has resulted in the construction of many infectious clones for the vast diversity of geminiviruses. In laboratory conditions, agrobacterium or occasionally biolistic methods are used to deliver viral DNA to the plant cell. However, not every delivered viral DNA will develop into an infection due to several reasons. In this manuscript, we review the factors that affect the success of geminivirus infectious clones. Factors affecting virus infection including the methods of inoculating in vitro-generated viral DNA constructs are often neglected, leading to failed virus infections and drawing wrong conclusions. Deciding exactly where on the plant to inoculate, what age of plant, and what agrobacterium strain are all examples of variables which may influence an infection. We find that stem injections of agrobacterium into young seedlings with an optical density at 600 nm (OD

Indexed as

GeminiviridaePlant DiseasesAgrobacteriumDNA, ViralDNA, ViralAgrobacterium infiltrationBegomovirusBiolisticPlant immunityVirus transmission

Identifiers

PMID40758195
PMCPMC12321669

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.