Evidence map›Paper›PMID 40757819›Full record

ArticleBiomarkers in medicine2025

Integrating cell-free DNA methylation of SEPT9 and SFRP2 into a machine learning model for early diagnosis of HCC.

Dong Wang, Zhihao Dai, Minghua Bai, Dong Liu, Yanru Feng, Quanquan Sun, Tong Zhang, Liang Han, Rui Wang, Ji Zhu and 2 more

Abstract read
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Article in Biomarkers in medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Dong WangMedical College, Hebei University of Engineering, Handan, Hebei, China.
Zhihao DaiSchool of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China.
Minghua BaiDepartment of Radiation Oncology, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, China.
Dong LiuDepartment of Radiation Oncology, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, China.
Yanru FengDepartment of Radiation Oncology, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, China.
Quanquan SunDepartment of Radiation Oncology, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, China.
Tong ZhangBioinformatics Development Department, Virtue Diagnostics (Suzhou)Co., Ltd, Suzhou, Jiangsu, China.
Liang HanBioinformatics Development Department, Virtue Diagnostics (Suzhou)Co., Ltd, Suzhou, Jiangsu, China.
Rui WangDepartment of Radiation Oncology, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, China.
Ji ZhuDepartment of Radiation Oncology, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, China.
Weifeng HongDepartment of Radiation Oncology, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, China.
Weiwei LiZhejiang Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHepatocellular carcinoma (HCC), a primary contributor to cancer-associated mortality, necessitates enhanced early detection. This study evaluated machine learning models that merge methylated SEPTIN9 (SEPT9) and secreted frizzled-related protein 2 (SFRP2) within circulating cell-free DNA (cfDNA) to detect HCC.

methodsA cohort of 165 healthy volunteers, 24 precancerous patients of HCC and 112 HCC patients were divided into training and validation sets. Methylated SEPT9 and SFRP2 (mSEPT9/mSFRP2) were detected using real-time PCR. Based on those methylation biomarkers and/or conventional biomarkers (CEA, AFP, CA125, and CA19-9), six machine learning algorithms, including Random Forest (RF), were employed to establish models for the training set. Models were evaluated for area under the ROC curve (AUC), sensitivity, and specificity, and subsequently validated in the validation set.

resultsThe RF model outperformed other models. In training, it achieved an AUC of 0.834 (95% CI: 0.745-0.923), exhibiting 69.3% sensitivity and 80.6% specificity for the methylation-specific signature group (mSS group: mSEPT9/mSFRP2). In validation, the RF model for the mSS group showed an AUC of 0.865 (95% CI: 0.811-0.946), with 85.4% sensitivity and 71.4% specificity.

conclusionsThe RF-based model integrating mSEPT9/mSFRP2 in cfDNA can be a promising approach for HCC diagnosis.

Indexed as

Carcinoma, HepatocellularCell-Free Nucleic AcidsDNA MethylationEarly Detection of CancerLiver NeoplasmsMachine LearningMembrane ProteinsSeptinsAdultAgedBiomarkers, TumorCase-Control StudiesFemaleHumansMaleMiddle AgedBiomarkers, TumorCell-Free Nucleic AcidsMembrane ProteinsSecreted Frizzled-Related ProteinsSEPTIN9 protein, humanSeptinsSFRP2 protein, humanDNA methylationearly diagnosisHepatocellular carcinomaSEPTIN9SFRP2

Identifiers

PMID40757819
PMCPMC12416165

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