Evidence map›Paper›PMID 40754853›Full record

ArticleGut microbes2025

Single cell viral tagging of

Danielle E Campbell, Xiaofen Wu, Lindsey R Hall, Dylan Lawrence, Jerome M Molleston, Lawrence A Schriefer, Lindsay Droit, James S Weagley, Benjamin S Olson, Joanna Rimmer and 3 more

Abstract read
In one paragraph

Article in Gut microbes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Gut virome dynamics: from commensal to critical player in health and disease.Nature reviews. Gastroenterology & hepatology · 2026
    Review
  5. Phage Therapy forAntibiotics (Basel, Switzerland) · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Danielle E CampbellDepartment of Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, MO, USA.ORCID 0000-0002-4072-3874
Xiaofen WuDepartment of Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, MO, USA.
Lindsey R HallDepartment of Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, MO, USA.ORCID 0000-0002-6012-9899
Dylan LawrenceDepartment of Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, MO, USA.ORCID 0000-0001-6919-8308
Jerome M MollestonEdison Family Center for Genome Sciences & Systems Biology, Washington University School of Medicine, St. Louis, MO, USA.ORCID 0000-0003-3702-8317
Lawrence A SchrieferDepartment of Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, MO, USA.ORCID 0000-0001-6865-8683
Lindsay DroitDepartment of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA.ORCID 0000-0002-5771-1490
James S WeagleyDepartment of Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, MO, USA.ORCID 0000-0001-5050-773X
Benjamin S OlsonDepartment of Molecular Microbiology, Division of Biology and Biomedical Sciences, Washington University School of Medicine, St. Louis, MO, USA.ORCID 0000-0002-5440-1315
Joanna RimmerDivision of Gastroenterology Addenbrooke's Hospital and Department of Medicine, University of Cambridge, Cambridge, UK.
Miles ParkesDivision of Gastroenterology Addenbrooke's Hospital and Department of Medicine, University of Cambridge, Cambridge, UK.ORCID 0000-0002-6467-0631
Scott A HandleyEdison Family Center for Genome Sciences & Systems Biology, Washington University School of Medicine, St. Louis, MO, USA.ORCID 0000-0002-2143-6570
Megan T BaldridgeDepartment of Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, MO, USA.ORCID 0000-0002-7030-6131

Funding

Infectious Diseases/Basic Microbial Pathogenic MechanismsT32AI007172 · NIAID · WASHINGTON UNIVERSITY · PI Daniel E. Goldberg, JENNIFER A PHILIPS · 1985 to 2026
$13.0M
Computational and Experimental Resources for Virome Analysis in Inflammatory Bowel Disease (CERVAID)RC2DK116713 · NIDDK · WASHINGTON UNIVERSITY · PI WANG, DAVID · 2019 to 2023
$8.9M
INSTITUTIONAL TRAINING GRANT IN GENOMIC SCIENCET32HG000045 · NHGRI · WASHINGTON UNIVERSITY · PI MICHAEL R BRENT, Barak A Cohen · 1997 to 2026
$8.4M
Pediatric Gastroenterology Research Training ProgramT32DK077653 · NIDDK · WASHINGTON UNIVERSITY · PI PHILLIP I TARR · 2007 to 2026
$5.8M
Functional characterization of viral-bacterial-human interactions during antimicrobial and inflammatory perturbations across different lifespansU01AT012998 · NCCIH · WASHINGTON UNIVERSITY · PI Megan T Baldridge, Gautam Dantas · 2024 to 2026
$5.3M
Pediatric Infectious Diseases and Immunity Training ProgramT32AI106688 · NIAID · WASHINGTON UNIVERSITY · PI Megan Anne Cooper, DAVID HUNSTAD · 2014 to 2026
$1.9M
NCCIH NIH HHS U01 AT012998NHGRI NIH HHS T32 HG000045NIAID NIH HHS T32 AI007172NIAID NIH HHS T32 AI106688NIDDK NIH HHS RC2 DK116713NIDDK NIH HHS T32 DK077653
6 · The paper itself

Abstract

The associations of the gut microbiome and virome with human health and disease are increasingly numerous and clear. The mechanistic roles of bacteriophages (phages) in the microbiome, however, are especially unclear, as their cultivation is exceedingly difficult and their diversity so immense. We use viral tagging (VT), a technique wherein fluorescently stained uncultivated viruses are allowed to adsorb to host cells and then host cells are singly sorted. This method identifies interacting phage-bacteria pairs to better sample and characterize the phages in human stool samples from healthy and inflammatory bowel disease (IBD)-affected patients. First, we apply VT to uncultivated bacteria from a healthy human sample, demonstrating far-reaching ability to observe diverse bacteria and phages alike. We also use VT with a cultured

Indexed as

BacteriophagesFaecalibacterium prausnitziiSingle-Cell AnalysisFecesGastrointestinal MicrobiomeHumansInflammatory Bowel DiseasesViromeBacteriophageFaecalibacterium prausnitziihuman gut microbiomehuman gut viromeinflammatory bowel diseasephageviral tagging

Identifiers

PMID40754853
PMCPMC12323421

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.