ArticleJournal of orthopaedic surgery and research2025
PiRNA hsa_pir_018849 promotes the proliferation and invasion of osteosarcoma cells by regulating the stability of CCN5 mRNA.
Article in Journal of orthopaedic surgery and research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- piR-27222 alleviates dexamethasone-induced osteoporosis by inhibiting ferroptosis through modulating WWP1.Journal of orthopaedic surgery and research · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
backgroundOsteosarcoma is a primary malignant bone tumor and that is predominantly found in adolescents and young adults, and it often occurs during periods of rapid bone growth. The precise specific etiology of osteosarcoma remains largely unknown. Piwi-interacting RNAs (piRNAs) are a class of small noncoding RNAs that are typically 24 to 31 nucleotides long, and they have potential therapeutic applications in diseases.
methodsSmall RNA sequencing and qPCR were initially used to detect differentially expressed piRNAs in the MG63 osteosarcoma cell line and in osteosarcoma tissues. The expression of hsa_piR_018849 in osteosarcoma cells was modulated using hsa_piR_018849 mimics and inhibitors. The impact of hsa_piR_018849 on the proliferation of osteosarcoma cells was assessed using CCK-8 and colony formation assays. Flow cytometry was used to investigate the effects of hsa_piR_018849 on osteosarcoma cell apoptosis. Cell scratch and Transwell assays were utilized to evaluate the impact of hsa_piR_018849 on the migration and invasion of osteosarcoma cells. mRNA sequencing was conducted to elucidate the mechanisms by which hsa_piR_018849 regulates osteosarcoma cell functions.
resultsOverexpression of hsa_piR_018849 promoted the proliferation, migration, and invasion of osteosarcoma cells while inhibiting apoptosis. Conversely, knockdown of hsa_piR_018849 suppressed osteosarcoma cell proliferation, migration, and invasion but promoted osteosarcoma cell apoptosis. Hsa_piR_018849 facilitated the proliferation of osteosarcoma cells in vivo. The expression levels of CCN5 were reduced by hsa_piR_018849. Hsa_piR_018849 reduced the stability of CCN5 by binding to the coding sequence (CDS) region of CCN5 mRNA.
conclusionPiRNA hsa_piR_018849 regulates the function of osteosarcoma cells by targeting and inhibiting the expression of CCN5.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.