ArticleBiophysical journal2025
Investigating the effects of temporal correlation on accurate molecular brightness determination.
Article in Biophysical journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Number and brightness, and photon counting histogram analyses, are powerful techniques for measuring stoichiometry in living cells. However, the accuracy of the results can be compromised by data temporal correlation, which increases with shorter acquisition times and slower molecular dynamics. Through simulations, we demonstrate that failing to account for data temporal correlation systematically underestimates the true molecular brightness. To overcome this limitation, we introduce the mixed assays to evaluate brightness analysis method, which decorrelates the data, allowing for accurate calculation of molecular brightness and achieving better precision with fewer data points.
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