Evidence map›Paper›PMID 40751220›Full record

ArticleJournal of animal science and biotechnology2025

Intestinal microbiota composition and bile salt hydrolase activity in fast and slow growing broiler chickens: implications for growth performance and production efficiency.

Hye Won Kim, Na Kyung Kim, Patricia G Wolf, Kristoffer Brandvold, Joshua M Rehberger, Tom G Rehberger, Ryan N Dilger, Alexandra H Smith, Roderick I Mackie

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In one paragraph

Article in Journal of animal science and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hye Won KimDepartment of Animal Sciences, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA.
Na Kyung KimDepartment of Animal Sciences, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA.
Patricia G WolfCollege of Health and Human Sciences, Purdue University, West Lafayette, IN, 47907, USA.
Kristoffer BrandvoldDepartment of Nutrition and Exercise Physiology, Elson S. Floyd College of Medicine, Washington State University, Spokane, WA, 99202, USA.
Joshua M RehbergerArm & Hammer Animal and Food Production, Waukesha, WI, 53186, USA.
Tom G RehbergerArm & Hammer Animal and Food Production, Waukesha, WI, 53186, USA.
Ryan N DilgerDepartment of Animal Sciences, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA.
Alexandra H SmithArm & Hammer Animal and Food Production, Waukesha, WI, 53186, USA.
Roderick I MackieDepartment of Animal Sciences, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA. r-mackie@illinois.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBody weight is an important indicator of the overall health and production efficiency in broiler chickens. In broiler houses, body weight of chicks is variable despite the same genetics, hatching and feeding practices within a production system. The objective of this study was to investigate the intestinal microbiota and bile salt hydrolase (BSH) activity in slow and fast growing broiler chickens, which belonged to the 10

methodsA total of 300 Ross 308 broiler chickens (100 per cohort from three independent cohorts) were selected and mucosal samples from the jejunum, ileum, and cecum were collected at day of arrival, 11 and 25 (n = 450). Then, bacterial counts, 16S rRNA amplicon sequencing, species specific real-time qPCR, as well as BSH activity were analyzed.

resultsResults of bacterial counts showed no significant difference between slow and fast growing cohorts (P > 0.05), but they tended to be higher in the slow growing chickens in all measured bacterial groups in cecum. The 16S rRNA amplicon sequencing revealed higher relative abundance of E. coli-Shigella (71.3%-79.8%) at day of arrival, while the most abundant microorganisms at d 25 was Candidatus Arthromitus (slow: 44.5%; fast: 27.4%) in small intestine. qPCR results indicated significant differences in bacterial populations between the slow and fast growing chickens, especially higher total bacteria, Enterococcus, and Clostridium cluster I in the slow growing chickens at d 25. BSH activity was higher in the slow growing chickens than the fast growing chickens [slow: 0.476 ΔOD/protein (μg/mL); fast: 0.258 ΔOD/protein (μg/mL); P < 0.0001], and correlation analysis highlighted associations between BSH activity, body weight, feed intake, body weight gain, and bacterial counts.

conclusionsWe postulate that high total bacteria and Enterococcus abundance are associated with high BSH activity, impacting low feed intake and body weight gain, ultimately resulting in separation into slow and fast growing birds. The findings of this study contribute to understanding the relationship between gut microbiota, BSH activity, and host physiology in broiler chickens, with potential implications for poultry production.

Indexed as

Bile salt hydrolase activityBroiler chickensFast growingGut microbiotaSlow growing

Identifiers

PMID40751220
PMCPMC12317501

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.