ArticleBMC musculoskeletal disorders2025
Temperature-based protection of biochemical and biomechanical characteristics during 25 kGy
Article in BMC musculoskeletal disorders, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundWhile prior studies have investigated cryopreservation, cross-linkers, and radical scavengers for tendon irradiation protection, optimal implementation protocols remain underexplored. We specifically evaluated temperature-dependent efficacy in preserving human tibialis posterior allograft tendons during 25kgy γ-irradiation.
methodsTwenty-four human tibialis posterior tendons were irradiated at -70 °C, 0 °C and room temperature. Collagen integrity was assessed through: (1) HE/Masson-stained fiber arrangement, (2) Sirius Red-polarized collagen periodicity, (3) hydroxyproline quantification. Biomechanical properties including elastic modulus and maximum stress were measured via tensile testing.
resultsIn the - 70 °C group, the structure and morphology of collagen fibers were significantly better than in the 0 °C group. At -70 ºC, collagen damage was 46.27% and 50.03% lower compared to 0ºC (P = 0.016) and RT (P < 0.004). The maximum stress of -70 °C (68.19 ± 30.11 MPa) is superior to that at 0 °C (36.58 ± 12.00 MPa) and RT (41.27 ± 16.34 MPa) (P < 0.01). The histological results based on fiber continuity, crimp integrity, and staining uniformity revealed superior collagen organization at -70 °C (7.2 ± 0.8) versus 0 °C (4.1 ± 1.1, P = 0.016) and RT (3.9 ± 1.3, P < 0.004). Hydroxyproline release was reduced by 46.27% (0 °C) and 50.03% (RT) in the - 70 °C group.
conclusionsDeep cryogenic preservation (-70 °C) demonstrates enhanced protective effects over conventional refrigeration for γ-irradiated tendon allografts, suggesting temperature optimization as critical in clinical processing protocols.
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