ArticleBMC microbiology2025
Screening and identification of host factors interacting with the nucleocapsid protein of SARS-CoV-2 omicron variant using the yeast two-hybrid system.
Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
1 citing paper in PubMed.
- Peptide Aptamers: Innovative Design and Applications in Pathogen Detection.Chembiochem : a European journal of chemical biology · 2026Review
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Authors and funding
11 authors.
Funding
Abstract
backgroundThe nucleocapsid protein (N protein) of SARS-CoV-2 is highly conserved in viral evolution and serves as the primary structural protein in viral infection, being the most abundant in viral particles. The N protein is highly immunogenic and plays a key role in the processes of viral infection and replication, making it of significant research value in both basic studies and clinical applications.
resultsTo further investigate the functions of SARS-CoV-2 N protein, the Matchmaker Gold Yeast Two-Hybrid System was used to identify potential interacting partners of the N protein in human peripheral blood mononuclear cells (PBMCs). Through this approach, we identified 11 host proteins that might interact with the SARS-CoV-2 N protein. We further validated the interaction between the N protein and two host proteins, RNF2 and ARL15, which showed the highest positive clone rates at the cellular level. We also predicted the critical amino acid residues mediating the interaction of the N protein with RNF2 or ARL15. Additionally, we explored the impact of these two host proteins on coronavirus replication. Functional analysis of all the 11 host proteins revealed their involvement in ribosome biogenesis, antigen processing and presentation, as well as various signaling pathways such as JAK-STAT.
conclusionsThis study further enriches the understanding of interactions between the SARS-CoV-2 N protein and host, providing important theoretical insights for the deeper understanding of viral pathogenesis and the development of antiviral strategies.
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