Evidence map›Paper›PMID 40751001›Full record

ArticleNature chemical biology2025

Directed evolution of LaccID for cell surface proximity labeling and electron microscopy.

Song-Yi Lee, Heegwang Roh, David Gonzalez-Perez, Mason R Mackey, Daniel Hoces, Colleen N McLaughlin, Chang Lin, Stephen R Adams, Khanh Nguyen, Keun-Young Kim and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature chemical biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
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  5. Singlet oxygen spills sterol secrets.Nature chemical biology · 2026
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  6. Review
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  9. AbioRxiv : the preprint server for biology · 2026
    Article
  10. Article
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  14. Review
  15. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

18 authors.

Song-Yi Lee *Department of Genetics, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0001-9091-8242
Heegwang Roh *Department of Chemistry, Stanford University, Stanford, CA, USA.
David Gonzalez-PerezDepartment of Biocatalysis, Institute of Catalysis, ICP-CSIC, Madrid, Spain.ORCID http://orcid.org/0000-0001-9613-4705
Mason R MackeyDepartment of Neurosciences, University of California, San Diego, La Jolla, CA, USA.
Daniel HocesDepartment of Genetics, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-1451-5166
Colleen N McLaughlinDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA.
Chang LinDepartment of Genetics, Stanford University, Stanford, CA, USA.
Stephen R AdamsDepartment of Pharmacology, University of California, San Diego, La Jolla, CA, USA.
Khanh NguyenBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Keun-Young KimDepartment of Neurosciences, University of California, San Diego, La Jolla, CA, USA.
David J LuginbuhlDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA.
Liqun LuoDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA.
Namrata D UdeshiBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0001-5312-1402
Steven A CarrBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-7203-4299
Rogelio A Hernández-LópezDepartment of Genetics, Stanford University, Stanford, CA, USA.
Mark H EllismanDepartment of Neurosciences, University of California, San Diego, La Jolla, CA, USA.
Miguel AlcaldeDepartment of Biocatalysis, Institute of Catalysis, ICP-CSIC, Madrid, Spain.ORCID http://orcid.org/0000-0001-6780-7616
Alice Y TingDepartment of Genetics, Stanford University, Stanford, CA, USA. ayting@stanford.edu.ORCID http://orcid.org/0000-0002-8277-5226

Funding

An Encyclopedia of the Adipose Tissue Secretome to Identify Mediators of Health and DiseaseRC2DK129961 · NIDDK · ROCKEFELLER UNIVERSITY · PI CHAIT, BRIAN T, COHEN, PAUL · 2021 to 2025
$9.2M
RIGERR: Resources for Investigating Genetic and Epigenetic Regulation of Renal DiseaseRC2DK129964 · NIDDK · UNIVERSITY OF ARIZONA · PI MINGYU LIANG · 2022 to 2026
$4.9M
Cell surface protein dynamics in neural circuit assemblyK99DC021195 · NIDCD · STANFORD UNIVERSITY · PI MCLAUGHLIN, COLLEEN · 2024 to 2025
$257k
National Science Foundation (NSF) NeuroNex UTA20-000889NIDCD NIH HHS K99 DC021195NIDCD NIH HHS L30 DC006982NIDDK NIH HHS RC2 DK129961NIDDK NIH HHS RC2 DK129964U.S. Department of Health & Human Services | National Institutes of Health (NIH) RC2DK129964
6 · The paper itself

Abstract

Enzymes that oxidize aromatic substrates have been harnessed for cell-based technologies including proximity labeling and electron microscopy; however, they are associated with drawbacks such as the need for toxic H

Indexed as

Cell MembraneDirected Molecular EvolutionLaccaseMicroscopy, ElectronAnimalsHumansOxidation-ReductionStaining and LabelingLaccase

Identifiers

PMID40751001
PMCPMC12643941

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.