ArticleBMC microbiology2025
3-Methyl pentanoic acid suppress gray mold disease potentially targeting cell-wall integrity (CWI) and mitogen-activated protein kinase (MAPK) pathways in Botrytis cinerea.
Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- 2-Ethylhexanol inhibit Botrytis cinerea by interfering in sugar and amino acid metabolism.NPJ science of food · 2026Article
- Recent advances in microbial production of odd-chain fatty acids.World journal of microbiology & biotechnology · 2026Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBotrytis cinerea, a notorious fungal pathogen, is responsible for causing grey mold in fruits and vegetables during the postharvest period. The use of environmentally friendly natural compounds presents a viable alternative to synthetic fungicides for managing postharvest diseases. 3-methyl pentanoic acid (3MP), a short-chain fatty acid widely employed as a flavoring agent and in agrochemicals, has not been explored for its potential in controlling postharvest diseases or its underlying mechanisms of action.
resultsThis study aimed to evaluate the antifungal activity and elucidate the potential mechanisms of action of 3MP against B. cinerea. Specifically, the effects of 3MP on mycelial growth, spore germination, disease development in tomatoes and strawberries fruit, cell viability, cell membrane integrity, and the expression of key genes (Chs1, Bmp1, Bmp3, and Sak1) associated with cell wall integrity (CWI) and mitogen-activated protein kinase (MAPK) signaling in B. cinerea were investigated. Results showed that mycelial growth, sporulation, spore germination, germ tube elongation, of B. cinerea were significantly suppressed by 3MP in a dose dependent manner. In vivo tests demonstrated that 3MP effectively controlled postharvest grey mold caused by B. cinerea on tomatoes and strawberries at concentrations of 12 μL/L. Fluorescein diacetate (FDA) and propidium iodide (PI) staining assays indicated that 3MP disrupted membrane integrity and impaired cell viability in B. cinerea. Additionally, the expression levels of CWI and MAPK-related genes Chs1, Bmp1, Bmp3 and Sak1 were significantly altered following 3MP treatment.
conclusion3MP represents a promising natural alternative to commercial fungicides or a lead compound for developing new biofungicide to control grey mold disease during the postharvest period. These results provide a theoretical basis for elucidating the underlying antifungal mechanism of 3MP against B. cinerea.
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