Evidence map›Paper›PMID 40750655›Full record

ArticleScientific reports2025

Methodic aspects of influenza and respiratory syncytial virus detection in raw wastewater and presence in treatment plants in southeastern Germany.

Michael Geissler, Helene Berndt, Ella Herberger, Konrad Wilms, Roger Dumke

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Michael GeisslerInstitute of Medical Microbiology and Virology, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
Helene BerndtInstitute of Medical Microbiology and Virology, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
Ella HerbergerInstitute of Medical Microbiology and Virology, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
Konrad WilmsInstitute of Medical Microbiology and Virology, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
Roger DumkeInstitute of Medical Microbiology and Virology, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany. roger.dumke@tu-dresden.de.ORCID http://orcid.org/0000-0001-6351-8817

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

During the COVID-19 pandemic, wastewater-based epidemiology (WBE) has been developed as an additional tool to follow epidemiological trends in the catchment area of treatment plants. Meanwhile, further viral agents of respiratory infections were included in monitoring programs. However, differences in sample processing may impair the results comparison among studies. With identical virus strains, we investigated different concentration methods, RNA isolation kits and primer/probe combinations for detection of influenza virus (IV) A/B and respiratory syncytial virus (RSV) A/B. For procedure optimization, virus enrichment and RNA extraction methods were found to be of relevance. Conversely, the detection step was identified to have a relatively low influence. Using standardized protocols, 24 h composite samples from eight wastewater treatment plants (WWTP) in southeast Germany (2x/week) were analyzed (January 2024 to December 2024) for IVA/B and RSV-A/B. The following rates of virus-positive samples were determined: 20.5% (IVA including 2.2% H1N1pdm09), 4.5% (IVB), 32.6% (RSV-A) and 2.4% (RSV-B). IV and RSV detections showed a matching trend when compared to reported cases. This study contributes to a better understanding of factors influencing the detection of IV and RSV in wastewater as well as in the epidemiological significance of virus monitoring in WWTPs.

Indexed as

Influenza, HumanRespiratory Syncytial VirusesRespiratory Syncytial Virus, HumanWastewaterCOVID-19GermanyHumansRespiratory Syncytial Virus InfectionsRNA, ViralRNA, ViralWastewaterInfluenza virusMonitoringRespiratory syncytial virusVirus detectionWastewaterWastewater-based epidemiology

Identifiers

PMID40750655
PMCPMC12317091

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.