Evidence map›Paper›PMID 40750648›Full record

ArticleScientific reports2025

Improved conservation of callus and rhizome microcuttings of Podophyllum hexandrum germplasm using the slow growth storage approach.

Zahoor Khan, Bushra Khan, Syed Tanveer Shah, Javaid Iqbal, Abdul Basit, Monsif Ur Rehman, Mian Afaq Ahmad, Muhammad Fahim, Muhammad Farhan Saeed, Árpád Székely and 1 more

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Zahoor KhanDepartment of Environmental Sciences, University of Peshawar, Peshawar, KP, 25130, Pakistan.
Bushra KhanDepartment of Environmental Sciences, University of Peshawar, Peshawar, KP, 25130, Pakistan.
Syed Tanveer ShahDepartment of Agriculture, Faculty of Biological and Health Sciences, Hazara University, Mansehra, KP, 21120, Pakistan.
Javaid IqbalDepartment of Environmental Sciences, University of Lakki Marwat, Lakki Marwat, KP, 28420, Pakistan.
Abdul BasitFloricultural Biotechnology Lab, Department of Horticultural Science, Kyungpook National University, Daegu, 41566, South Korea.
Monsif Ur RehmanDepartment of Agriculture, Faculty of Biological and Health Sciences, Hazara University, Mansehra, KP, 21120, Pakistan.
Mian Afaq AhmadInstitute of Biotechnology and Genetic Engineering, The University of Agriculture-Peshawar, Peshawar, Khyber Pakhtunkhwa, 25130, Pakistan.
Muhammad FahimCentre for Omic Sciences, Islamia College University Peshawar, Peshawar, KP, 25130, Pakistan.
Muhammad Farhan SaeedDepartment of Environmental Sciences, COMSATS University Islamabad, Vehari-Campus, Vehari, 61100, Pakistan.
Árpád SzékelyResearch Centre for Irrigation and Water Management, Institute of Environmental Sciences, Hungarian University of Agriculture and Life Sciences, Anna-Liget str. 35, Szarvas, 5540, Hungary. szekely.arpad@uni-mate.hu.
Aftab JamalDepartment of Soil and Environmental Sciences, Faculty of Crop Production Sciences, The University of Agriculture, Peshawar, 25130, Pakistan. aftabses98@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Podophyllum hexandrum Royle (syn. Podophyllum emodi Wall.), commonly known as Himalayan mayapple, is an endangered medicinal plant recognized as the primary natural source of podophyllotoxin, a potent compound with anticancer and antiviral properties. In this study, we developed an optimized protocol for the long-term preservation of P. hexandrum germplasm using a slow growth storage (SGS) technique, successfully preserving the viability and genetic stability of both callus and rhizome cuttings. In vitro cultured callus and rhizome microcuttings of P. hexandrum were conserved using the slow growth storage (SGS) technique in Murashige and Skoog (MS) medium under cold conditions (5 °C), supplemented with different concentrations of sucrose, mannitol, and sorbitol in combination with calcium pantothenate and spermidine, to induce slow growth and maintain tissue viability. It was observed that sorbitol (5.5%) combined with spermidine (2 mg L

Indexed as

PodophyllumPreservation, BiologicalRhizomeBerberidaceaePlants, MedicinalCalcium pantothenateIn vitro conservationOsmotic regulatorsRhizome microcuttingsSlow growth storage (SGS)SpermidineTissue viability

Identifiers

PMID40750648
PMCPMC12316991

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.