Evidence map›Paper›PMID 40748049›Full record

ArticleeLife2025

Phage-displayed synthetic library and screening platform for nanobody discovery.

Baolong Xia, Ah-Ram Kim, Feimei Liu, Myeonghoon Han, Emily Stoneburner, Stephanie Makdissi, Francesca Di Cara, Stephanie E Mohr, Aaron Ring, Norbert Perrimon

Abstract read
In one paragraph

Article in eLife, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Immuno-positron emission tomography in cardiovascular disease: Advances, challenges, and opportunities.Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Baolong Xia *Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, United States.ORCID https://orcid.org/0000-0003-2536-0267
Ah-Ram Kim *Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, United States.ORCID https://orcid.org/0000-0001-9597-6759
Feimei LiuDepartment of Immunobiology, Yale School of Medicine, Boston, United States.
Myeonghoon HanDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, United States.
Emily StoneburnerDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, United States.
Stephanie MakdissiDepartment of Microbiology and Immunology, Dalhousie University, Halifax, Canada.
Francesca Di CaraDepartment of Microbiology and Immunology, Dalhousie University, Halifax, Canada.
Stephanie E MohrDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, United States.ORCID https://orcid.org/0000-0001-9639-7708
Aaron RingDepartment of Immunobiology, Yale School of Medicine, Boston, United States.
Norbert PerrimonDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, United States.ORCID https://orcid.org/0000-0001-7542-472X

Funding

Functional genomics resources for the Drosophila - TR&D3P41GM132087 · NIGMS · HARVARD MEDICAL SCHOOL · PI MOHR, STEPHANIE E · 2019 to 2023
$6.8M
Drosophila models of human mitochondrial diseasesR24OD035556 · OD · HARVARD MEDICAL SCHOOL · PI NORBERT PERRIMON · 2023 to 2026
$3.3M
National Research Foundation 2021R1A6A3A14039622NIGMS NIH HHS P41 GM132087NIH HHS NIH 5R24OD035556NIH HHS NIH NIGMS P41 GM132087NIH HHS R24 OD035556
6 · The paper itself

Abstract

Nanobodies, single-domain antibodies derived from camelid heavy-chain antibodies, are known for their high affinity, stability, and small size, which make them useful in biological research and therapeutic applications. However, traditional nanobody generation methods rely on camelid immunization, which can be costly and time-consuming, restricting their practical feasibility. In this study, we present a phage-displayed synthetic library for nanobody discovery. To validate this approach, we screened nanobodies targeting various

Indexed as

Peptide LibrarySingle-Domain AntibodiesAnimalsCell Surface Display TechniquesDrosophilaDrosophila ProteinsDrosophila ProteinsPeptide LibrarySingle-Domain Antibodiesbiochemistrychemical biologyD. melanogasternanobody screenphage-displaysecreted proteinsynthetic library

Identifiers

PMID40748049
PMCPMC12316454

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.