ArticleVirulence2025
A human intestinal organoid derived from fetal human colon cells model for studying enteroviral pathogenesis.
Article in Virulence, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human enteroviruses, including enterovirus 71 (EV71), cause hand, foot, and mouth disease (HFMD) and may lead to severe neurological diseases in infants. Enteroviruses first infect the gastrointestinal tract and then spread to the main organs, such as the liver, lungs, heart, and brain. Human intestinal organoids (HIOs) provide a physiologically relevant model for studying enterovirus infections. Unlike traditional two-dimensional (2D) monolayer cultures, HIOs maintain complex epithelial cell diversity and three-dimensional (3D) architecture, allowing for a more accurate representation of in vivo viral-host interactions. In this study, we developed efficient and stable HIOs based on fetal human primary colon cells using the 3D culture system. We discriminated cultured HIOs containing goblet, enteroendocrine, and Paneth cells, and examined the replication efficiency of enteroviruses in HIOs compared to 2D monolayer cultures. HIOs were infected with enteroviruses (EV71, coxsackievirus B3, echovirus 6), and viral replication was assessed using molecular and imaging techniques, which exhibit a higher level of dynamic viral replication in HIOs than in 2D culture. The replication level of enteroviruses increased about 10-fold in HIOs with the virus titre in HIOs was 5-10 times higher than that in 2D cell cultures (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.