ArticleMicrobial cell factories2025
Coordinated synthesis of aromatic amino acid derived pigments in yeast via global transcriptional engineering.
Article in Microbial cell factories, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Color-associated metabolic divergence and nutritional variation in colored cauliflower curds.Food chemistry: X · 2026Article
- Recent advances in metabolic engineering for microbial production of L-tyrosine.World journal of microbiology & biotechnology · 2026Review
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6 authors.
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Abstract
backgroundRational metabolic pathway engineering is capable of boosting upstream flux towards downstream synthesis of target products, such as aromatic amino acid derivatives. However, coordinated synthesis of multiple downstream derivatives faces difficulty of combinatorial optimization of cellular metabolism.
resultsWe developed a strategy combining metabolic engineering optimization with the global transcriptional regulation of transcription factors (TFs) Spt15p and Gcn4p to optimize the synthesis of aromatic amino acid derivatives in yeast. It is verified that the special mutants of these TFs can respectively improve the biosynthesis of betaxanthin, a tyrosine derived edible pigment. Comparative transcriptome analysis shows that significant transcriptional tuning occurs in glycolysis, pentose phosphate pathway, aromatic amino acid synthesis pathways, etc. In addition, global transcriptional engineering is proved to enhance the coordinated biosynthesis of both tyrosine derived pigment betaxanthin and tryptophan derived pigment violacein by more than 50%. Finally, we obtain an optimized production of 208 mg/L betaxanthin in yeast cells by flask fermentation.
conclusionsOur strategy supplies an effective way to optimize the coordinated synthesis of two structurally divergent pigments downstream of the common aromatic amino acid pathway.
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