Evidence map›Paper›PMID 40745264›Full record

ArticleBMC genomics2025

Genome-wide identification and expression pattern analysis of NAC family in Taxus yunnanensis and the TyuNAC30 role in paclitaxel production.

Jiangtao Fan, Yong Wang, Mengke Dou, Qiuhong Hu, Qing Li, Shengmei Gan, Mingwei Jing, Gang Wang, Xiong Huang

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Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

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7citing papers in PubMed
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1 · What the graph read from it

What it found

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3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Genome-Wide Analysis ofCurrent issues in molecular biology · 2026
    Article
  4. Article
  5. Article
  6. Recent advances inFrontiers in plant science · 2026
    Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jiangtao FanForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chendu, 610000, China.
Yong WangSichuan Mt. Emei Forest Ecosystem National Observation and Research Station, Sichuan Agricultural University, Chendu, 610000, China. wangyong2015@sicau.edu.cn.
Mengke DouForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chendu, 610000, China.
Qiuhong HuForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chendu, 610000, China.
Qing LiForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chendu, 610000, China.
Shengmei GanForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chendu, 610000, China.
Mingwei JingForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chendu, 610000, China.
Gang WangForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chendu, 610000, China. wanggang@sicau.edu.cn.
Xiong HuangForest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chendu, 610000, China. huangxiongcaf@163.com.

Funding

Central Finance Forestry and Grassland Science and Technology Promotion Demonstration Project [2023]9)Research Initiation Fund X H, 031/2222996047Sichuan Province Innovative Talent Funding Project for Postdoctoral Fellows 2322999070
6 · The paper itself

Abstract

backgroundThe NAC transcription factor family represents a large group of plant-specific genes involved in various biological processes, including secondary metabolite biosynthesis, responses to biotic and abiotic stresses, and hormone signaling. Taxus yunnanensis, a well-known medicinal plant, is the natural source of the anticancer diterpene paclitaxel. Despite the biological importance of NAC transcription factors, no comprehensive genome-wide study of the NAC gene family has been conducted in T. yunnanensis.

resultsIn this study, 44 NAC genes were identified from the T. yunnanensis genome. Gene structure analysis revealed that the number of exons ranged from 1 to 7, with most genes containing 2 to 3 exons. Chromosomal mapping showed an uneven distribution of TyuNACs across the 11 chromosomes. Duplication type analysis indicated that dispersed duplication is the primary mechanism driving the expansion of the TyuNACs, accounting for the largest proportion (24 genes), followed by tandem (12 genes) and proximal duplications (8 genes); no whole-genome or segmental duplication events were detected. Cis-regulatory element analysis suggested that TyuNACs are involved in key biological processes such as development, light response, stress adaptation, and hormone signaling. Expression profiling revealed diverse tissue-specific expression patterns, with most TyuNACs highly expressed in bark. Moreover, qRT-PCR analysis demonstrated that several TyuNACs respond to methyl jasmonate (MeJA) treatment. Yeast one-hybrid and related assays revealed that TyuNAC30 functions as a negative regulator of TyuDBTNBT, a key enzyme gene in the paclitaxel biosynthetic pathway.

conclusionThis study presents the comprehensive genome-wide characterization of the NAC transcription factor family in T. yunnanensis. The findings underscore the predominant role of dispersed duplication in the expansion of the TyuNAC family, with additional contributions from tandem and proximal duplications. Importantly, TyuNAC30 was identified as a negative regulator of TyuDBTNBT, suggesting a key role in the transcriptional regulation of paclitaxel biosynthesis. These findings offer valuable insights for further exploration of the functional roles of NAC genes, particularly their involvement in the regulatory mechanisms underlying paclitaxel biosynthesis.

Indexed as

Gene Expression Regulation, PlantMultigene FamilyPaclitaxelPlant ProteinsTaxusTranscription FactorsChromosome MappingGene DuplicationGene Expression ProfilingGenome, PlantPhylogenyPaclitaxelPlant ProteinsTranscription FactorsExpression patternGenome-wide identificationNAC family genesPaclitaxel biosynthesisTaxus yunnanensis

Identifiers

PMID40745264
PMCPMC12312540

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.