Evidence map›Paper›PMID 40745014›Full record

ArticleScientific reports2025

An effective approach to cartilage regeneration using antler stem cell-conditioned medium.

Jue Zhou, Baorui Xing, Qianqian Guo, Jianwei Zhao, Xunsheng Li, Datao Wang, Zhigang Yue, Jinpeng Lv, Yidi Jiang, Hongmei Sun

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jue Zhou *Chinese Academy of Agricultural Sciences, Institute of Special Animal and Plant Sciences, 4899 Jingyue St., Changchun, 130112, Jilin, China.
Baorui Xing *Jinan Key Laboratory of Oral Tissue Regeneration, Shandong Provincial Health Commission Key Laboratory of Oral Diseases and Tissue Regeneration, Jinan Stamotological Hospital, Jinan, 250001, Shandong , China.
Qianqian GuoInstitute of Antler Science and Product Technology, Changchun Sci-Tech University, Changchun, 130112, Jilin, China.
Jianwei ZhaoChinese Academy of Agricultural Sciences, Institute of Special Animal and Plant Sciences, 4899 Jingyue St., Changchun, 130112, Jilin, China.
Xunsheng LiChinese Academy of Agricultural Sciences, Institute of Special Animal and Plant Sciences, 4899 Jingyue St., Changchun, 130112, Jilin, China.
Datao WangChinese Academy of Agricultural Sciences, Institute of Special Animal and Plant Sciences, 4899 Jingyue St., Changchun, 130112, Jilin, China.
Zhigang YueChinese Academy of Agricultural Sciences, Institute of Special Animal and Plant Sciences, 4899 Jingyue St., Changchun, 130112, Jilin, China.
Jinpeng LvChinese Academy of Agricultural Sciences, Institute of Special Animal and Plant Sciences, 4899 Jingyue St., Changchun, 130112, Jilin, China.
Yidi JiangChinese Academy of Agricultural Sciences, Institute of Special Animal and Plant Sciences, 4899 Jingyue St., Changchun, 130112, Jilin, China.
Hongmei SunChinese Academy of Agricultural Sciences, Institute of Special Animal and Plant Sciences, 4899 Jingyue St., Changchun, 130112, Jilin, China. sunhongmei@caas.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Articular cartilage has a low capacity for self-regeneration. Therefore, stem cell therapy has been proposed and is gaining momentum. Antler can self-repair and its cartilaginous tissue can grow at an unprecedented rate (Up to 2 cm/day) and antler regeneration is based on antler stem cells. Therefore, we predicted that antler stem cells or their paracrine factors might be a good source for promoting cartilage repair. In this study, we prepared conditioned medium from antler stem cells (ASC-CM) and evaluated its effect. We implanted the medium into rat cartilage defects and assessed its capacity to promote cartilage repair. The protein composition of ASC-CM was analyzed using via DIA assay. ASC-CM can strongly promote chondrocyte proliferation in vitro; it significantly up-regulates the expression of chondrogenesis-related genes (Aggrecan, Col II, and Sox-9) and promotes glycosaminoglycan formation and type II collagen deposition in cartilaginous tissue; meanwhile up-regulate the apoptosis suppressor gene NAMPT and down-regulate apoptosis gene BAX. In vivo, cartilage defect in rats was significantly repaired using ASC-CM. The compositions of ASC-CM were got and some key proteins such as S100A4 were identified by bioinformatics analysis. The ASC-CM can promote the proliferation of rat-chondrocytes, maintain the chondrocyte phenotype, and inhibit apoptosis of rat-chondrocytes. Therefore, it can promote cartilage repair.

Indexed as

AntlersCartilage, ArticularRegenerationStem CellsAnimalsApoptosisCell ProliferationChondrocytesChondrogenesisCulture Media, ConditionedMaleRatsRats, Sprague-DawleyCulture Media, ConditionedAntler stem cellsCartilage defectCartilage regenerationCell proliferationConditioned medium

Identifiers

PMID40745014
PMCPMC12314024

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.