Evidence map›Paper›PMID 40744727›Full record

ArticleCold Spring Harbor protocols2026

A Surgical Method for Oocyte Injection and CRISPR-Cas9 Mutagenesis in

Christina E Sabin, Sukhada P Samudra, Anna L Iouchmanov, Amber L Rittgers, James D Lauderdale, Douglas B Menke

Abstract read
In one paragraph

Article in Cold Spring Harbor protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Cold Spring Harbor protocols · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Christina E Sabin *Neuroscience Division of the Biomedical and Translational Sciences Institute, University of Georgia, Athens, Georgia 30602, USA.ORCID http://orcid.org/0000-0002-3188-393X
Sukhada P Samudra *Department of Genetics, University of Georgia, Athens, Georgia 30602, USA.ORCID http://orcid.org/0000-0002-7006-8970
Anna L IouchmanovDepartment of Genetics, University of Georgia, Athens, Georgia 30602, USA.ORCID http://orcid.org/0000-0002-5064-1290
Amber L RittgersDepartment of Genetics, University of Georgia, Athens, Georgia 30602, USA.ORCID http://orcid.org/0000-0001-8805-9207
James D LauderdaleNeuroscience Division of the Biomedical and Translational Sciences Institute, University of Georgia, Athens, Georgia 30602, USA.ORCID http://orcid.org/0000-0001-7503-0528
Douglas B MenkeDepartment of Genetics, University of Georgia, Athens, Georgia 30602, USA dmenke@uga.edu.ORCID http://orcid.org/0000-0002-7109-1451

Funding

T32 Predoctoral training grant in geneticsT32GM007103 · NIGMS · UNIVERSITY OF GEORGIA (UGA) · PI DYER, KELLY A · 1985 to 2021
$5.9M
T32 Predoctoral Training Grant in GeneticsT32GM142623 · NIGMS · UNIVERSITY OF GEORGIA · PI Kelly A Dyer, Mary Grace Goll · 2022 to 2026
$2.1M
Collaborative Research: EDGE FGT: DEVELOPMENT OF FUNCTIONAL GENETIC TOOLS IN GECKOSR01HG013006 · NHGRI · MARQUETTE UNIVERSITY · PI GAMBLE, ANTHONY B, MENKE, DOUGLAS BAY · 2023 to 2025
$1.1M
NHGRI NIH HHS R01 HG013006NIGMS NIH HHS T32 GM007103NIGMS NIH HHS T32 GM142623
6 · The paper itself

Abstract

Squamates, the taxon that comprises lizards and snakes, are a diverse assemblage of reptiles represented by more than 11,000 described species. Studies of gene function in squamates, however, have remained very limited, largely due to the lack of established genetic tools and suitable experimental systems. A major challenge for the development of CRISPR-based gene editing in these reptiles is that the isolation of fertilized oocytes or single-celled embryos is impractical for most species, given that fertilization occurs internally, the females of many species can store sperm, and simple methods for detecting ovulation are lacking. To overcome these challenges, we have developed a unique surgical approach in the brown anole lizard

Indexed as

CRISPR-Cas SystemsGene EditingLizardsMicroinjectionsMutagenesisOocytesAnimalsFemale

Identifiers

PMID40744727
PMCPMC12376173

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.