ArticleActa odontologica latinoamericana : AOL2025
Feasibility of using saliva for Cytomegalovirus detection and genotyping in pediatric hematopoietic stem cell transplant recipients.
Article in Acta odontologica latinoamericana : AOL, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Human Cytomegalovirus (HCMV) is a major viral pathogen that causes severe complications in immunosuppressed individuals, particularly hematopoietic stem cell transplant recipients. In these patients, Cytomegalovirus has been associated with gastroenteritis, pneumonia, hepatitis, and even graft-versus-host disease, and a possible relationship has been identified between Cytomegalovirus genotypes and clinical course, complications and outcome. Early detection of Cytomegalovirus infection or reactivation is important, and previous findings show that it could potentially be evaluated in saliva, where HCMV causes asymptomatic viral shedding. Since saliva can be collected easily and safely, it is important to evaluate its potential for HCMV detection and genotyping, especially in pediatric patients who are receiving hematopoietic stem cell transplantation.
aimThe purpose of this study was to evaluate the feasibility of using saliva to detect and genotype HCMV in a cohort of pediatric hematopoietic stem cell transplant recipients (HSCTR). MATERIALS AND
methodThis study was conducted at Fundación Hospital Pediátrico la Misericordia, in Bogota, Colombia. Stimulated saliva samples were collected once a week and subjected to HCMV detection by qualitative PCR and genotyping by nested PCR followed by sequencing. Finally, a phylogenetic tree was constructed.
resultsTwenty patients were enrolled, and 105 saliva samples were collected, of which 29 were positive for HCMV. Twelve patients had at least one positive sample. The gB1 genotype was identified with no coinfection with any other genotype. Phylogenetic analysis showed that some saliva samples were closer to the sequence reported for the Towne laboratory strain, while others were closer to the Merlin strain, with slight differences between them.
conclusionsIt was demonstrated that saliva can be used to detect and genotype Cytomegalovirus in pediatric transplant recipients, and that sample collection is easy, with no risk of bleeding or discomfort in the pediatric patients evaluated.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.