Evidence map›Paper›PMID 40739791›Full record

ArticleMagnetic resonance in medicine2025

Nuclear spin hyperpolarization of pyruvate enables longitudinal monitoring of treatment response in intestinal tumor organoids.

Josh P Peters, Hang Xiang, Charbel D Assaf, Farhad Haj Mohamad, Philip Rosenstiel, Stefan Schreiber, Jan-Bernd Hövener, Konrad Aden, Andrey N Pravdivtsev

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Magnetic resonance in medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Josh P PetersSection Biomedical Imaging, Molecular Imaging North Competence Center, Department of Radiology and Neuroradiology, University Hospital Schleswig-Holstein, Kiel University, Kiel, Germany.ORCID https://orcid.org/0000-0003-1019-4067
Hang XiangInstitute of Clinical Molecular Biology, Kiel University, Kiel, Germany.
Charbel D AssafSection Biomedical Imaging, Molecular Imaging North Competence Center, Department of Radiology and Neuroradiology, University Hospital Schleswig-Holstein, Kiel University, Kiel, Germany.ORCID https://orcid.org/0000-0003-1968-2112
Farhad Haj MohamadSection Biomedical Imaging, Molecular Imaging North Competence Center, Department of Radiology and Neuroradiology, University Hospital Schleswig-Holstein, Kiel University, Kiel, Germany.ORCID https://orcid.org/0009-0005-2238-5639
Philip RosenstielInstitute of Clinical Molecular Biology, Kiel University, Kiel, Germany.ORCID https://orcid.org/0000-0002-9692-8828
Stefan SchreiberDepartment of Internal Medicine I, University Hospital Schleswig-Holstein, Kiel, Germany.
Jan-Bernd HövenerSection Biomedical Imaging, Molecular Imaging North Competence Center, Department of Radiology and Neuroradiology, University Hospital Schleswig-Holstein, Kiel University, Kiel, Germany.ORCID https://orcid.org/0000-0001-7255-7252
Konrad AdenInstitute of Clinical Molecular Biology, Kiel University, Kiel, Germany.ORCID https://orcid.org/0000-0003-3482-7316
Andrey N PravdivtsevSection Biomedical Imaging, Molecular Imaging North Competence Center, Department of Radiology and Neuroradiology, University Hospital Schleswig-Holstein, Kiel University, Kiel, Germany.ORCID https://orcid.org/0000-0002-8763-617X

Funding

Bundesministerium für Bildung und Forschung (BMBF) 01ZX1915ABundesministerium für Bildung und Forschung (BMBF) 01ZX1915CDeutsche Forschungsgemeinschaft (DFG) 469366436Deutsche Forschungsgemeinschaft (DFG) 527469039Deutsche Forschungsgemeinschaft (DFG) 555951950Deutsche Forschungsgemeinschaft (DFG) EXC2167Deutsche Forschungsgemeinschaft (DFG) FOR5042Deutsche Forschungsgemeinschaft (DFG) HO-4602/2-2Deutsche Forschungsgemeinschaft (DFG) HO-4602/3Deutsche Forschungsgemeinschaft (DFG) RU 5042EKFS #2019_A09EKFS 2020_EKCS.11European Regional Development Fund (ERDF) 122-09-053
6 · The paper itself

Abstract

purposeColorectal cancer, a leading cause of death in the Western world, is increasingly affecting younger populations. The Warburg effect, characterized by enhanced lactate production, is a hallmark of this cancer type. Although

methodsThis study uses hyperpolarized [1-

resultsHyperpolarized NMR revealed a 6.6-fold reduction (p < 0.05) in lactate production in rapamycin-treated organoids, indicating suppressed metabolic activity. This method also detected alanine and bicarbonate metabolism, highlighting its sensitivity. Unlike traditional methods that destroy cellular integrity, hyperpolarization enabled repetitive, noninvasive metabolic assessments.

conclusionHyperpolarized [1-13C]pyruvate combined with NMR enables noninvasive, longitudinal monitoring of tumor metabolism in intestinal organoids while preserving cell viability and recultivation potential, bridging preclinical and clinical applications and affirming the method's potential for targeted metabolic imaging as a novel diagnostic and treatment control approach in cancer medicine.

Indexed as

Colorectal NeoplasmsIntestinal NeoplasmsMagnetic Resonance ImagingOrganoidsPyruvic AcidAnimalsCarbon IsotopesDisease Models, AnimalIntestinesLactic AcidMagnetic Resonance SpectroscopyMiceCarbon IsotopesLactic AcidPyruvic Acidhyperpolarizationintestinal carcinogenesisorganoidspyruvaterapamycin

Identifiers

PMID40739791
PMCPMC12501731

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.