Evidence map›Paper›PMID 40739759›Full record

ArticlePlant communications2025

HiMT: An integrative toolkit for assembling organelle genomes using HiFi reads.

Shuyuan Tang, Yanyang Liang, Fengqi Wu, Ya Wu, Jiawei Li, Lvna Lin, Junting Feng, Aiping Luan, You Wang, Chunxiang Xu and 5 more

Abstract read
In one paragraph

Article in Plant communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. The Complete Mitogenome ofJournal of fungi (Basel, Switzerland) · 2026
    Article
  2. A Compact Multipartite Mitogenome ofInternational journal of molecular sciences · 2026
    Article
  3. Article
  4. Article
  5. Mitochondrial DNA. Part B, Resources · 2026
    Article
  6. The complete chloroplast genome assembly ofMitochondrial DNA. Part B, Resources · 2026
    Article
  7. Newly assembled mitochondrial genomes ofFrontiers in plant science · 2026
    Article
  8. Comparative organellar genomics ofFrontiers in plant science · 2026
    Article
  9. Article
  10. Article
  11. Assembly and sequence analysis of the chloroplast genome ofMitochondrial DNA. Part B, Resources · 2026
    Article
  12. Complete chloroplast genome and phylogenetic analysis ofMitochondrial DNA. Part B, Resources · 2026
    Article
  13. The complete mitochondrial genome ofFrontiers in plant science · 2026
    Article
  14. The complete plastome sequence ofMitochondrial DNA. Part B, Resources · 2026
    Article
  15. Article
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Shuyuan TangState Key Laboratory of Tropical Crop Breeding, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in South China, Ministry of Agriculture and Rural Affairs, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510640, China.
Yanyang LiangState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510640, China.
Fengqi WuState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510640, China.
Ya WuEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China.
Jiawei LiState Key Laboratory of Tropical Crop Breeding, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in South China, Ministry of Agriculture and Rural Affairs, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China.
Lvna LinState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510640, China.
Junting FengState Key Laboratory of Tropical Crop Breeding, Sanya Research Institute, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 572024, China.
Aiping LuanState Key Laboratory of Tropical Crop Breeding, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in South China, Ministry of Agriculture and Rural Affairs, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China.
You WangState Key Laboratory of Tropical Crop Breeding, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in South China, Ministry of Agriculture and Rural Affairs, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China.
Chunxiang XuState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510640, China.
Jiabao WangState Key Laboratory of Tropical Crop Breeding, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in South China, Ministry of Agriculture and Rural Affairs, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China; Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China.
Yongfeng ZhouState Key Laboratory of Tropical Crop Breeding, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in South China, Ministry of Agriculture and Rural Affairs, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China.
Rui XiaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510640, China.
Yi LiaoState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510640, China.
Chengjie ChenState Key Laboratory of Tropical Crop Breeding, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in South China, Ministry of Agriculture and Rural Affairs, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China. Electronic address: ccj@catas.cn.

Funding

Non-US Government Research Support type
6 · The paper itself

Abstract

Chloroplast and mitochondrial genomes, which coexist with the nuclear genome, are extensively used in gene function studies, evolutionary analyses, and targeted breeding. However, assembling these organelle genomes remains challenging due to frequent recombination events and abundant repetitive sequences. Although some assembly tools are available, many are difficult to install or require extensive parameter tuning and computational resources. To address these limitations, we present a high-fidelity, data-driven mitochondrial genome assembly toolkit (HiMT), a user-friendly, out-of-the-box software solution that enables one-click chloroplast and mitochondrial genome assembly using default parameters, particularly for plant species. HiMT automatically estimates read coverage depth and employs a fixed k-mer prefix strategy to minimize computational demands, making it suitable for use on standard laptops. Benchmark tests show that HiMT delivers complete assemblies with a high success rate, fast runtimes, and low hardware requirements. Additionally, HiMT features a graphical user interface (GUI) and generates interactive reports for assessing the quality of organelle genome assemblies. We anticipate that HiMT will facilitate high-quality plant mitochondrial genome research and significantly streamline organelle genome assembly workflows. To support the research community, HiMT is freely available to non-commercial users at https://github.com/tang-shuyuan/HiMT.

Indexed as

Genome, ChloroplastGenome, MitochondrialGenome, PlantSoftwarealgorithm optimizationgenome assemblymitochondriaplastidssoftware development

Identifiers

PMID40739759
PMCPMC12447420

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.