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ArticleInternational microbiology : the official journal of the Spanish Society for Microbiology2025

Microbes, smile for the picture: protocol and guide for capturing images and processing of microbial cultures with smartphones.

Jefferson Brendon Almeida Dos Reis, Sofia Coradini Schirmer, Chiara Tawane Lauriola, Gabriel Barbosa Dos Santos, Ana Julia Pereira Rodrigues, Gustavo Medeiros Januário, Helson Mario Martins do Vale, Danilo Batista Pinho

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In one paragraph

Article in International microbiology : the official journal of the Spanish Society for Microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jefferson Brendon Almeida Dos ReisDepartment of Phytopathology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, DF, Brazil. jeffersonalmeidareis@gmail.com.ORCID http://orcid.org/0000-0002-2768-214X
Sofia Coradini SchirmerPostgraduate Program in Ecology, Department of Zoology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, DF, Brazil.ORCID http://orcid.org/0000-0001-7200-7269
Chiara Tawane LauriolaDepartment of Phytopathology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, DF, Brazil.
Gabriel Barbosa Dos SantosDepartment of Phytopathology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, DF, Brazil.
Ana Julia Pereira RodriguesDepartment of Phytopathology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, DF, Brazil.
Gustavo Medeiros JanuárioDepartment of Phytopathology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, DF, Brazil.
Helson Mario Martins do ValeDepartment of Phytopathology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, DF, Brazil.
Danilo Batista PinhoDepartment of Phytopathology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, DF, Brazil.ORCID http://orcid.org/0000-0003-2624-302X

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 001
6 · The paper itself

Abstract

Photography has revolutionized science by enabling the documentation and analysis of phenomena, including microbial cultures, where high-quality images are essential for research. However, certain inherent qualities of objects, such as the reflective surfaces of Petri dishes and the characteristics of fungal colonies, often compromise image quality. This highlights the need for accessible, low-cost solutions. Here, we present "Microbes, smile for the picture" (MSFP), a practical, portable, and affordable protocol using smartphones and a handmade photo studio equipped with a ring light, enabling standardized, high-quality images of microbial colonies for scientific and outreach purposes. The aim of this work is to describe the procedure for constructing the photograph studio and a protocol for obtaining high-quality photos. We believe that images captured using our studio and protocol can enhance scientific reproducibility and improve image quality for research, illustration, and science communication. Additionally, this protocol has the potential to be applied in other fields, generating high-quality images for educational and science popularization purposes.

Indexed as

FungiImage Processing, Computer-AssistedMicrobiological TechniquesPhotographySmartphoneMicrobiologyMycologyPhotography in researchScientific imagingSmartphone photography

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.