Evidence map›Paper›PMID 40739431›Full record

ArticleMikrochimica acta2025

Electrochemical DNA biosensor based on a novel pyrrolidinyl peptide nucleic acid sequence immobilized on a graphene oxide/bovine serum albumin-modified electrode for the direct diagnosis of seed-borne pathogen.

Hendra Hendra, Wiphawee Leesutthiphonchai, Nattakorn Kuncharoen, Supot Kasem, Orawon Chailapakul, Tugba Ozer, Sakda Jampasa, Tiyakhon Chatnaparat

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Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hendra HendraTropical Agriculture, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand.
Wiphawee LeesutthiphonchaiDepartment of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand.
Nattakorn KuncharoenDepartment of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand.
Supot KasemDepartment of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand.
Orawon ChailapakulElectrochemistry and Optical Spectroscopy Center of Excellence (EOSCE), Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Tugba OzerDepartment of Bioengineering, Faculty of Chemical-Metallurgical Engineering, Yildiz Technical University, Istanbul, Türkiye.
Sakda JampasaFuturistic Science Research Center, School of Science, Walailak University, Nakhon Si Thammarat, 80160, Thailand. sakda.jam@wu.ac.th.
Tiyakhon ChatnaparatDepartment of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand. fagrtkc@ku.ac.th.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Xanthomonas oryzae pv. oryzae (Xoo) is a significant pathogen responsible for seed-borne diseases that negatively impact rice yields globally. Effective early detection methods for this pathogen, particularly those based on nucleic acid detection, are crucial for maintaining plant health. This study presented an electrochemical DNA biosensor that utilized a novel pyrrolidinyl peptide nucleic acid (acpcPNA) probe sequence derived from the wxoC gene, which encoded a putative glycosyltransferase, for sensitive, selective, and direct detection of Xoo in rice seed samples. The acpcPNA probe was designed, synthesized, and characterized using mass spectrometry and melting temperature analysis. It was then immobilized onto a graphene oxide-bovine serum albumin (GO/BSA) nanocomposite modified electrode to demonstrate its electrochemical sensing capabilities, as GO/BSA aided in stabilizing film formation, leading to an effective immobilization process. The formation of the probe with Xoo double-stranded DNA (dsDNA) via duplex invasion considerably reduced the redox signal response due to electrostatic repulsion and insulating properties. With the optimized conditions, the sensor demonstrated a linear detection range from 10 to 20 × 10

Indexed as

Biosensing TechniquesDNA, BacterialElectrochemical TechniquesPeptide Nucleic AcidsXanthomonasAnimalsCattleElectrodesGraphiteLimit of DetectionOryzaPlant DiseasesSeedsSerum Albumin, BovineDNA, Bacterialgraphene oxideGraphitePeptide Nucleic AcidsSerum Albumin, BovineElectrochemical DNA biosensorNanocompositePyrrolidinyl peptide nucleic acidSeed-borne diseaseXanthomonas oryzae pv. oryzae

Identifiers

PMID40739431

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.