Evidence map›Paper›PMID 40736890›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

DNA Sequencing and the Third-Generation Sequencing Revolution.

Jamie Harrison

Abstract readHistorical Article
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Jamie HarrisonMRC Centre for Medical Mycology, University of Exeter, Exeter, UK. J.W.Harrison@exeter.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Since the discovery of the structure of DNA in the 1950s and the invention of DNA sequencing in the 1970s, a huge number of scientific advances driven by breakthroughs made using sequencing technologies have been made. More recently, the advent and advances in second and third generation sequencing technologies have enabled new insights into genomic architecture and variation, and the emerging phenotype. In this chapter, I introduce a brief history of DNA sequencing, the emergence of third generation sequencing, Pac-Bio sequencing, Oxford Nanopore Technology, genome assembly challenges and approaches, and examples of notable genome assembly successes including from several bacterial species. I conclude with a synopsis of the current and upcoming topics driving the sequencing and genome assembly revolution.

Indexed as

High-Throughput Nucleotide SequencingSequence Analysis, DNAGenomicsHistory, 20th CenturyHistory, 21st CenturyHumansBacterial genomicsDNA sequencingGenome assembly

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.