Whole Mount Oligopaint Fluorescence In Situ Hybridization of Mouse Oocytes, Eggs, or Early-Stage Embryos.

Frances E Clark, Duilio M Z A Silva, Morgan Skinner, Leah F Rosin, Naomi L Greenberg, Takashi Akera

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

A Vertebrate Toxin-Antidote System That Sabotages Mouse Embryogenesis.bioRxiv : the preprint server for biology · 2026
Article

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Frances E ClarkCell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.

Duilio M Z A SilvaCell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.

Morgan SkinnerCell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.

Leah F RosinUnit on Chromosome Dynamics, Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.

Naomi L GreenbergCell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.

Takashi AkeraCell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA. takashi.akera@nih.gov.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluorescence in situ hybridization (FISH) to visualize DNA or chromosomes in cells is well recognized as a valuable and versatile technique. The recent development of Oligopaint FISH is particularly useful for studies of chromosome structure, function, and evolution. Here, we describe a series of protocols for applying the Oligopaint FISH technique to mouse oocytes, eggs, and early-stage embryos. We describe techniques to adhere these cell types to microscopy slides, improving the ease of applying the FISH technique. We also provide an option to combine this with immunofluorescence (IF).

Indexed as

Embryo, MammalianIn Situ Hybridization, FluorescenceOocytesOvumAnimalsFemaleMiceFISHMouse embryoMouse oocyteOligopaintZona pellucida

Identifiers

PMID40736792

DOI10.1159/000485283

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.