Evidence map›Paper›PMID 40736350›Full record

ArticleMicrobiology spectrum2025

High-resolution melting curve FRET-qPCR rapidly distinguishes

Nneka Vivian Iduu, Donna Raiford, Noah D Cohen, Kerstin K Landrock, Chengming Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Septic arthritis caused byASM case reports · 2026
    Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Nneka Vivian IduuMolecular Diagnostic Laboratory, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.
Donna RaifordDepartment of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.
Noah D CohenDepartment of Large Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.ORCID 0000-0002-0384-2903
Kerstin K LandrockDepartment of Large Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
Chengming WangMolecular Diagnostic Laboratory, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.ORCID 0000-0001-7874-8340

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We developed a fluorescence resonance energy transfer (FRET)-based real-time PCR (qPCR) assay coupled with high-resolution melting (HRM) analysis to differentiate IMPORTANCE: Accurately identifying the cause of respiratory infections in horses is essential for proper treatment and preventing outbreaks. This study introduces a rapid and reliable test that distinguishes between

Indexed as

Fluorescence Resonance Energy TransferHorse DiseasesReal-Time Polymerase Chain ReactionStreptococcal InfectionsStreptococcusStreptococcus equiAnimalsDNA, BacterialHorsesPolymorphism, Single NucleotideReproducibility of ResultsRNA, Ribosomal, 23SSensitivity and SpecificityTransition TemperatureWhole Genome SequencingDNA, BacterialRNA, Ribosomal, 23SFRET-qPCRhigh-resolution melting curve analysismolecular diagnosticsstranglesStreptococcus equi subsp. equiStreptococcus equi subsp. zooepidemicus

Identifiers

PMID40736350
PMCPMC12403774

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.