Evidence map›Paper›PMID 40735767›Full record

ArticleCirculation research2025

Bacterial Infections Shape Cardiac Macrophages' Response to Ischemia.

Simon Geissen, Alexander Hof, Elvina Santhamma Philip, Elena Wagner, Charlotte Schreiber, Kezia Singgih, Suchitra Narayan, Harshal Nemade, Linus Maximilian Hohendorff, Niklas Arno Roever and 12 more

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Article in Circulation research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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1 citing paper in PubMed.

  1. Review
4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Simon Geissen *Faculty of Medicine and University Hospital Cologne, Department III of Internal Medicine, Heart Center (S.G., A.H., E.S.P., E.W., C.S., K.S., S.N., H.N., L.M.H., N.A.R., P.S., M.M., H.W., S.B., F.F.H.), University of Cologne, Germany.ORCID 0000-0002-7835-4111
Alexander Hof *Faculty of Medicine and University Hospital Cologne, Department III of Internal Medicine, Heart Center (S.G., A.H., E.S.P., E.W., C.S., K.S., S.N., H.N., L.M.H., N.A.R., P.S., M.M., H.W., S.B., F.F.H.), University of Cologne, Germany.ORCID 0000-0001-6554-8892
Elvina Santhamma Philip *Faculty of Medicine and University Hospital Cologne, Department III of Internal Medicine, Heart Center (S.G., A.H., E.S.P., E.W., C.S., K.S., S.N., H.N., L.M.H., N.A.R., P.S., M.M., H.W., S.B., F.F.H.), University of Cologne, Germany.ORCID 0000-0001-6084-1977
Elena WagnerFaculty of Medicine and University Hospital Cologne, Department III of Internal Medicine, Heart Center (S.G., A.H., E.S.P., E.W., C.S., K.S., S.N., H.N., L.M.H., N.A.R., P.S., M.M., H.W., S.B., F.F.H.), University of Cologne, Germany.
Charlotte SchreiberFaculty of Medicine and University Hospital Cologne, Department III of Internal Medicine, Heart Center (S.G., A.H., E.S.P., E.W., C.S., K.S., S.N., H.N., L.M.H., N.A.R., P.S., M.M., H.W., S.B., F.F.H.), University of Cologne, Germany.ORCID 0009-0007-8476-3097
Kezia SinggihFaculty of Medicine and University Hospital Cologne, Department III of Internal Medicine, Heart Center (S.G., A.H., E.S.P., E.W., C.S., K.S., S.N., H.N., L.M.H., N.A.R., P.S., M.M., H.W., S.B., F.F.H.), University of Cologne, Germany.ORCID 0009-0004-8517-2078
Suchitra NarayanFaculty of Medicine and University Hospital Cologne, Department III of Internal Medicine, Heart Center (S.G., A.H., E.S.P., E.W., C.S., K.S., S.N., H.N., L.M.H., N.A.R., P.S., M.M., H.W., S.B., F.F.H.), University of Cologne, Germany.
Harshal NemadeFaculty of Medicine and University Hospital Cologne, Department III of Internal Medicine, Heart Center (S.G., A.H., E.S.P., E.W., C.S., K.S., S.N., H.N., L.M.H., N.A.R., P.S., M.M., H.W., S.B., F.F.H.), University of Cologne, Germany.ORCID 0000-0002-2704-181X
Linus Maximilian HohendorffFaculty of Medicine and University Hospital Cologne, Department III of Internal Medicine, Heart Center (S.G., A.H., E.S.P., E.W., C.S., K.S., S.N., H.N., L.M.H., N.A.R., P.S., M.M., H.W., S.B., F.F.H.), University of Cologne, Germany.
Niklas Arno RoeverFaculty of Medicine and University Hospital Cologne, Department III of Internal Medicine, Heart Center (S.G., A.H., E.S.P., E.W., C.S., K.S., S.N., H.N., L.M.H., N.A.R., P.S., M.M., H.W., S.B., F.F.H.), University of Cologne, Germany.
Patrik SchelemeiFaculty of Medicine and University Hospital Cologne, Department III of Internal Medicine, Heart Center (S.G., A.H., E.S.P., E.W., C.S., K.S., S.N., H.N., L.M.H., N.A.R., P.S., M.M., H.W., S.B., F.F.H.), University of Cologne, Germany.ORCID 0000-0001-5532-5265
Michelle HolthausFaculty of Medicine and University Hospital Cologne, Department of Cardiac Surgery, Heart Center (M.H., A.P.-G.), University of Cologne, Germany.ORCID 0000-0002-6207-1141
Adnana Paunel-GörgülüFaculty of Medicine and University Hospital Cologne, Department of Cardiac Surgery, Heart Center (M.H., A.P.-G.), University of Cologne, Germany.ORCID 0000-0001-6467-3544
Martin MollenhauerFaculty of Medicine and University Hospital Cologne, Department III of Internal Medicine, Heart Center (S.G., A.H., E.S.P., E.W., C.S., K.S., S.N., H.N., L.M.H., N.A.R., P.S., M.M., H.W., S.B., F.F.H.), University of Cologne, Germany.ORCID 0000-0002-3220-9269
Holger WinkelsFaculty of Medicine and University Hospital Cologne, Department III of Internal Medicine, Heart Center (S.G., A.H., E.S.P., E.W., C.S., K.S., S.N., H.N., L.M.H., N.A.R., P.S., M.M., H.W., S.B., F.F.H.), University of Cologne, Germany.ORCID 0000-0003-1333-2031
Norbert GerdesMedical Faculty and University Hospital, Department of Cardiology, Pulmonology and Vascular Medicine (N.G.), Heinrich-Heine-University, Düsseldorf, Germany.ORCID 0000-0002-4546-7208
Kat Folz-DonahueFACS & Imaging Core Facility, Max Planck Institute for Biology of Ageing, Cologne, Germany (K.F.-D., A.-L.S., C.K.).
Anna-Lena SchumacherFACS & Imaging Core Facility, Max Planck Institute for Biology of Ageing, Cologne, Germany (K.F.-D., A.-L.S., C.K.).ORCID 0000-0001-7739-486X
Christian KukatFACS & Imaging Core Facility, Max Planck Institute for Biology of Ageing, Cologne, Germany (K.F.-D., A.-L.S., C.K.).ORCID 0000-0003-1508-0229
Stephan BaldusFaculty of Medicine and University Hospital Cologne, Department III of Internal Medicine, Heart Center (S.G., A.H., E.S.P., E.W., C.S., K.S., S.N., H.N., L.M.H., N.A.R., P.S., M.M., H.W., S.B., F.F.H.), University of Cologne, Germany.ORCID 0000-0001-8259-1737
Timofei ZatsepinSouffle Therapeutics, Watertown, MA (T.Z.).ORCID 0000-0003-0030-9174
Friedrich Felix HoyerFaculty of Medicine and University Hospital Cologne, Department III of Internal Medicine, Heart Center (S.G., A.H., E.S.P., E.W., C.S., K.S., S.N., H.N., L.M.H., N.A.R., P.S., M.M., H.W., S.B., F.F.H.), University of Cologne, Germany.ORCID 0009-0002-3957-0302

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPatients with bacterial infections are at increased risk for subsequent cardiovascular events. Whether infections' effects on innate immune cells within the cardiovascular system influence subsequent pathologies remains unclear. Here, we explore cardiac myeloid cells' chronic adaptations to a preceding bacterial insult and implications for subsequent myocardial ischemia.

methodsWe used various flow cytometry protocols to assess cardiac immune cells, peripheral leukocytes, and hematopoietic stem and progenitor cells in bone marrow and spleen. A genetic fate-mapping model was used to determine cardiac macrophages' origin after bacteremia. Cardiac leukocytes were analyzed using single-cell RNA sequencing. Nanoparticle-mediated RNA interference was used to target macrophages in vivo.

resultsCardiac macrophage numbers increased sharply, and numerical changes alongside subset alterations persisted over time. Fate-mapping pointed toward local origin as the primary macrophage source after infection. Profiling macrophage heterogeneity using single-cell RNA sequencing, we identified two previously unknown subpopulations remaining after resolution of infection. While heightened metabolic activity was one subset's primary feature, the other displayed excessive chemotactic properties, which amplified cardiac leukocyte recruitment and inflammation after a subsequent ischemic injury. Targeting cardiac macrophages' surplus inflammatory activity after infection using nanoparticle-enabled, macrophage-directed RNA interference kept disproportionate subsequent ischemic inflammation at bay.

conclusionsBacteremia induces long-lasting changes in the cardiovascular system's innate immune cells' composition. This may amplify myocardial inflammation after a subsequent ischemic injury.

Indexed as

BacteremiaMacrophagesMyocardial IschemiaMyocardiumAnimalsDisease Models, AnimalImmunity, InnateMaleMiceMice, Inbred C57BLinfectionsinflammationmacrophagesmyeloid cellsmyocardial infarction

Identifiers

PMID40735767
PMCPMC12398350

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